利用生物信息学方法预测survivin拮抗肽的性质和功能  

PREDICTION OF FEATURES AND FUNCTION OF SURVIVIN ANTAGONISTIC PEPTIDES BY BIOINFORMATICAL METHODS

作　　者：窦长武[1] 田复明[1] 王晓娟[2] 高乃康[1] 武文元[1] 

机构地区：[1]内蒙古医学院附属医院神经外科,内蒙古呼和浩特010050 [2]内蒙古医学院免疫学教研室

出　　处：《内蒙古医学院学报》2007年第4期239-242,共4页Acta Academiae Medicinae Neimongol

基　　金：内蒙古自然科学基金资助项目(200408020902);内蒙古医学院附属医院重大项目资助项目(200405)

摘　　要：目的:利用生物信息学方法对survivin拮抗肽(SP-1～SP-8)进行理化性质的预测及相似性的比较,为拮抗肽的结构与功能研究提供预测和帮助。方法:应用ExPASy数据库分析短肽的Mr、等电点、疏水性等理化性质。利用Blastp和SCOP等数据库对survivin拮抗肽进行相似性比较。结果:经ExPASy数据库分析8条拮抗肽中有6条呈碱性,且大部分呈亲水性,半衰期由1.0～30.0h不等。Blastp分析表明有几种拮抗肽与RNA多聚酶,转录调控因子的活性中心部分序列相似。SCOP分析结果显示有几种拮抗肽与ras结合蛋白(RBD)和CAD结构域相似。Objective：To analyze and predict the physical and chemical characteristics of survivin antagonistic peptides （ SPs）, and to compare its similarity with other known proteins and to provide some evidence for further research on antagonistic peptides＇ structures and functions. Methods：Integrated bioinformatics database ExPASy was used to analyze molecular weight （Mr）, isoelectric point （ pI）, hydrophobicity and half life of SPs. Blastp and SCOP database were used to analyze the similarity with other known proteins. Results：It was found that 6 of the 8 SPs were rich in basic amino acids,. most of which were hydrophilic and their half life was 1.0 - 30.0 hours. Similar sequences were found between several peptides and RNA polymerase, transcriptional regulator by Blastp, between several peptides and ras binding protein （RBD） and CAD domain by SCOP. Conclusion： Bioinformatical methods are helpful in the study of polypeptide and prediction of protein structure and function as well as drug designing.

关 键 词：生物信息学 拮抗肽 结构与功能预测 

分 类 号：R735.7[医药卫生—肿瘤]