VEGF、ANG-1、ANG-2、TSP-1的表达与胆管细胞性肝癌血管生成和侵润转移的关系  

Expressions of VEGF,ANG-1,ANG-2 and TSP-1 in cholangiocellular carcinoma and relationship with tumor angiogenesis,differentiation,invasion and metastasis

作　　者：汤地[1] 周奇[2] 梁力建[1] 彭宝岗[1] 永野皓昭[3] 

机构地区：[1]中山大学附属第一医院肝胆外科,广东广州510080 [2]中山大学附属第一医院黄埔院区,广东广州510700 [3]大阪大学附属病院消化器外科

出　　处：《中国病理生理杂志》2007年第10期2046-2050,共5页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金博士科研启动资助项目(No.7300945)

摘　　要：目的:研究血管内皮生长因子(VEGF)、血管生成素-1(ANG-1)、血管生成素-2(ANG-2)、血小板反应蛋白-1(TSP-1)的表达与胆管细胞性肝癌(CCC)血管生成和侵润转移的关系。方法:对33例手术切除的CCC标本进行CD34、VEGF、ANG-1、ANG-2和TSP-1的免疫组化染色,研究VEGF、ANG-1、ANG-2、TSP-1的表达与胆管细胞性肝癌血管生成和肿瘤门静脉侵犯、肝内转移、淋巴结转移以及肿瘤分化水平之间的关系。结果:本组CCC的微血管密度(MVD)为(87.2±52.6)/mm2,VEGF、ANG-1、ANG-2和TSP-1的阳性率分别为75.6%、36.0%、57.6%和45.5%。VEGF和ANG-2的阳性表达与高MVD相关,TSP-1则与MVD负相关(P<0.01,P<0.05,P<0.01)。阳性TSP-1与肝内转移正相关(46.7%vs5.6%,P<0.05)。结论:CCC瘤内的血管新生活跃,VEGF和ANG-2的阳性表达与CCC血管生成正相关,TSP-1则与其负相关,TSP-1的阳性表达还与肝内转移相关,VEGF、ANG-1、ANG-2的表达与肿瘤的侵润转移未见显著相关。AIM： To investigate the angiogenesis status, the expression of vascular endothelial growth factor （ VEGF）, angiopoietin - 1 （ ANG - 1 ）, angiopoietin - 2 （ ANG - 2）, thrombospondin - 1 （ TSP - 1 ） in cholangiocellular carcinoma （CCC） and relationship with tumor angiogenesis, differentiation, invasion and metastasis. METHODS： 33 specimen of surgically resected CCC were investigated. Immunohistochemical staining of CD34, VEGF, ANG - 1, ANG -2 and TSP - 1 was carried out. RESULTS： The mean MVD was （87. 2 ±52. 6）/mm^2. VEGF positive expression was found in 75.6% cases; ANG - 1 positive expression was observed in 36% cases; ANG -2 positive was detected in 57.6% cases and 45.5% cases exhibited positive TSP - 1 expression. VEGF and ANG - 2 expressions were found to be associated with significant higher level of MVD （ P 〈 0. 01 and P;〈 0. 05, respectively）. TSP - 1 expression was found to be associated with significant low level of MVD （P 〈 0. 01 ）. Positive TSP - 1 expression was also found to be associated with higher level of intrahepatic metastasis （46. 7% vs 5.6%, P 〈 0. 05 ）. CONCLUSION： Considerable angiogenesis compared to other solid tumors can be observed in CCC. VEGF and ANG - 2 might play a proangiogenic role and TSP - 1 may play an inhibitory role. Although TSP - 1 may increase the intrahepatic metastasis of CCC, neither MVD levels nor the expression of VEGF, ANG - 1, or ANG - 2 is associated with tumor differentiation, invasion and metastasis.

关 键 词：肝肿瘤 血管内皮生长因子 血管生成素类 血小板反应蛋白1 肿瘤侵润 肿瘤转移 

分 类 号：R735.7[医药卫生—肿瘤]