^(188)Re-7E11C5.3抑制前列腺癌细胞增殖  

Antiproliferation effects of ^(188)Re labeled monoclonal antibody on prostate cancer cells in vitro

作　　者：陈维真[1] 张勇[1] 卢汉平[2] 梁昌盛[3] 谢瑶[4] 刘长征[2] 

机构地区：[1]中山大学附属第三医院核医学科,广东广州510630 [2]中山大学基础医学院实验核医学教研室,广东广州510080 [3]中山大学基础医学实验教学中心,广东广州510080 [4]中山大学基础医学院人体解剖学教研室,广东广州510080

出　　处：《中国病理生理杂志》2007年第10期2051-2053,共3页Chinese Journal of Pathophysiology

基　　金：广东省科技计划资助项目(No.2003B30103)

摘　　要：目的:探讨铼-188(188Re)标记前列腺特异膜抗原单克隆抗体7E11C5.3(188Re-7E11C5.3)对前列腺癌细胞系LNCaP的体外抑制作用。方法:采用2-巯基乙醇直接还原法制备188Re-7E11C5.3标记物,纸层析法测定标记率和放化纯度,直线回归外推法测定免疫活性分数。四唑盐(MTT)法测定其体外细胞毒作用。结果:188Re-7E11C5.3的标记率为(93.16±2.18)%,放化纯度为(95.62±0.48)%,免疫活性分数为(74.86±1.86)%。188Re-7E11C5.3对LNCaP细胞的生长抑制作用明显强于188Re标记的正常鼠IgG(mIgG)和游离188ReO4-,其半数有效抑制浓度(IC50)分别为(23.38±3.73)×107Bq/L,(59.21±8.02)×107Bq/L和(68.89±10.91)×107Bq/L。结论:188Re-7E11C5.3能有效抑制体外培养LNCaP细胞的增殖,可用于前列腺癌的放射免疫治疗。AIM： To investigate the effect of 188 Re labeled monoclonal antibody on prostatic specific membrane antigen 7E1 1 C5.3, radioimmunotherapy for the treatment of human prostate cancer cell line LNCaP in vitro. METHODS ： 188 Re -7E1 1 C5.3 was prepared by direct 2 - mercaptoethanol reduction method. Labeling efficiency and radiochemical purity was measured by paper chromatography. Immunoreactive fraction was determined by linear extrapolation. Cytotoxicity to LNCaP cells was determined by MTT assay. RESULTS： The labeling yield of 188 Re -7E1 1 C5.3 was （93. 16 ±2. 18 ）% , the radiochemical purity was （95.62 ±0. 48）% , and the immunoreactive fraction was （74. 86± 1.86）%. The inhibitory effect of 188Re -7E11C5.3 on cell proliferation of LNCaP cells was significantly higher than that of 188Re - mlgG or 188 ReO4^-. The 50% inhibitory doses （ IC50 ） of 188Re - 7E1 1 C5.3, 188 Re - mIgG, and lss ReO4^- were （23.38 ±3.73 ） ×10^7 Bq/L, （59. 21 ±8.02） × 10^7 Bq/L and （68.89 ±10. 91 ） × 10^7 Bq/L, respectively. CONCLUSION： 188Re - 7E11C5.3 can effectively inhibit the growth of in vitro cultured prostate cancer cells and shows much potential for prostate cancer radioimmunotherapy.

关 键 词：前列腺肿瘤 放射免疫疗法 抗体 单克隆 前列腺特异膜抗原 铼-188 

分 类 号：R737.25[医药卫生—肿瘤]