枸杞原汁、枸杞多糖诱导人正常肝细胞L-O2及卵巢癌细胞株SKOV3、HO8910凋亡的实验研究  

Experimental study on the apoptosis of human normal hepatic cell line L-O2 and ovarian carcinoma cell line SKOV3,HO8910 induced by Fructus Lycii juice and Lycium barbarum polysaccharide (LBP)

作　　者：王晋 王月玲[2] 

机构地区：[1]西安交大医学院,陕西西安710061 [2]西安交通大学第一附属医院,陕西西安710061

出　　处：《现代肿瘤医学》2007年第11期1542-1545,共4页Journal of Modern Oncology

摘　　要：目的:对比研究枸杞原汁、枸杞多糖对人正常肝细胞L-O2及人卵巢癌细胞株SKOV3、HO8910的生长抑制,及诱导凋亡的作用及其机制。方法:枸杞原汁、枸杞多糖作用L-O2、SKOV3、HO8910,用MTT法检测细胞生长抑制率,流式细胞仪检测细胞凋亡率并分析细胞周期分布的变化。结果:枸杞原汁组:L-O2、SKOV3、HO8910生长抑制率分别为-58%,-49%,-84%;L-O2、HO8910凋亡细胞含量明显少于空白对照组,正常细胞数明显多于空白对照组;枸杞原汁对L-O2、HO8910无细胞周期阻滞,各期细胞分别较对照组均匀;枸杞多糖组:枸杞多糖(500,1000,2000)mg/L作用下,L-O2生长抑制率分别为28%,31%,20%;SK-OV3为-1%,40%,35%,HO8910为13%,48%,41%;不同浓度LBP对L-O2及HO8910均有诱导凋亡作用,凋亡细胞数以1000mg/L为最大;1000mg/L组L-O2、HO8910细胞周期均阻滞于S期,S期细胞比例高于对照组。结论:枸杞原汁在体外对L-O2、SKOV3、HO8910有促进生长作用,且对HO8910的作用最大;对L-O2、HO8910无诱导细胞凋亡作用。枸杞多糖在体外对L-O2、SKOV3、HO8910都有抑制生长作用,且对HO8910作用最大;对L-O2、HO8910有诱导细胞凋亡作用,以浓度1000mg/L为最大,并未表现出剂量依赖效应。枸杞多糖诱导细胞发生S期阻滞,并且诱导S期细胞发生凋亡是其体外抑制细胞生长的可能机制之一。To study the growth inhibition of Fructus Lycii juice and LBP on normal hepatic cell line L-O2 and ovarian cancer cell line SKOV3 ,HO8910 ,and investigate the apoptosis mechanism of three cell lines induced by Fructus Lycii juice and Lycium barbarum polysaccharide （LBP）. Methods： After treated by Fructus Lycii juice and LBP, the growth inhibition of L-O2, SKOV3 and HO8910 cells was determined using Methyl thiazolyl tet- razolium （MTT） assay, the rate of apoptosis and phase distribution of cell cycle of L-O2, HO8910 were detected by flow cytometry （FCM）. Results. Fructus Lycii juice group： the rates of growth inhibition of L-O2, SKOV3 and HO8910 cell lines were -58%, -49%, -84% respectively; it could not induce apoptosis on L-O2 and HO8910 cells; there were not cell phase blocks on L-O2 and HO8910 cell lines. Lycium barbarum polysaccharide group： The rates of growth inhibition of LBP（500,1000,2000）mg/L on L-O2 were 28% ,31% ,20% respectively; the rates on SKOV3 were - 1% ,40% ,35% respectively; the rates on HO8910 were 13% ,48% ,41% respectively;it could induce apoptosis on L-O2 and HO8910 cells, the number of apoptosis cells was most at 1000mg/L than that of 500mg/L and 2000mg/L; the rate of cells in S phase was more than that of contrasting group respectively, the cell phases of L-O2 and HO8910 cell lines were arrested in S phase. Conclusion： Fructus Lycii juice could promote the growth L-O2, SKOV3 and HO8910 in vitro. The effect on HO8910 cell line was strongest, it could not induce apoptosis on L-O2 and HO8910 cells. Lycium barbarum polysaccharide could inhibit the growth of L-O2, SKOV3, and HO8910 cells in vitro. Theeffect on HO8910 was strongest. It could induce apoptosis on L-O2 and HO8910 cells. The effect is not dose - dependent. The cell phases of L-O2 and HO8910 cell lines were arrested in S phase.

关 键 词：人体正常肝细胞 卵巢肿瘤细胞 枸杞原汁 枸杞多糖(LBP) 凋亡 

分 类 号：R730[医药卫生—肿瘤]