上皮型钙粘附素和γ-连环素在非小细胞肺癌中的表达及临床意义  

A clinical study on the expression of E-cadherin and γ-catenin in human non-small cell lung cancer

作　　者：岳峰涛[1] 陈健[2] 梁景仁[2] 王鸿雁[2] 

机构地区：[1]复旦大学附属上海市第五人民医院胸外科,上海200240 [2]西安交通大学第一医院,陕西西安710061

出　　处：《现代肿瘤医学》2007年第11期1571-1574,共4页Journal of Modern Oncology

摘　　要：目的:探讨上皮型钙粘附素(E-cadherin,E-cad)和γ-连环素(γ-catenin,γ-cat)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及与NSCLC的发生、发展及预后的关系。方法:应用免疫组化PicTureTM通用型二歩法,观察43例NSCLC常规石蜡包埋组织及7例正常肺组织中的E-cad和γ-cat蛋白的表达。结果:在43例NSCLC中,E-cad和γ-cat的异常表达率分别为58.1%和67.4%,显著高于正常肺组织(P<0.05、P<0.01)。伴有淋巴结转移及TNMⅢ期组E-cad异常表达率明显高于无淋巴结转移组及TNMⅠ、Ⅱ期组(P<0.01);腺癌组织及伴有淋巴结转移组γ-cat异常表达率明显高于鳞癌组织及无淋巴结转移组(P<0.05)。E-cad和γ-cat异常表达者生存时间明显少于正常表达者(中位生存时间,16vs35个月,P<0.01;20vs32个月,P<0.05)。Cox比例风险模型分析显示TNM分期、淋巴结转移情况和E-cad表达情况为影响预后的独立因素(P<0.05)。E-cad表达情况与γ-cat表达情况显著相关(r=0.416,P=0.007)。联合分析E-cad、γ-cat发现,E-cad和γ-cat两种蛋白均正常表达组与只有E-cad或γ-cat一种蛋白正常表达组相比淋巴结转移发生率较低(P<0.01)、生存期较长(中位生存时间,45vs20个月,P<0.01)。结论:E-cad和γ-cat蛋白表达与NSCLC的发生、发展及预后关系密切,可作为评估NSCLC进展及预后判断的有价值的指标。联合检测两种蛋白有助于提高判断NSCLC淋巴结转移情况及预后分析的准确性。To investigate the role of E -cadherin（E -cad） and γ-catenin（γ-cat） in development and prognosis of non - small cell lung cancer （ NSCLC）. Methods： Forty - three specimens of resected, paraffin embedded NSCLC and 7 cases of normal lung tissues were examined by immunohistochemical technique for the expres- sion of E - cad and γ-cat. Results ： Of the 43 NSCLC cases, the abnormal rates of E - cad and γ- cat protein were 58.1% and 67.4% respectively, which were significantly higher than normal lung specimens （ P 〈 0. 05, P 〈 0. Ol ）. The abnormal expression of E - cad was significantly correlated with lymph node metastasis （ P 〈 0.01 ） and TNM stage （P 〈 0.01 ）. The abnormal expression of γ-cat was more frequently found in the adenocarcinomas than in the squamous cell carcinomas （ P 〈 0. 05 ） and was significantly correlated with lymph node metastasis （ P 〈 0. 05 ）. The survival time was significantly shorter in patients with E - cad or γ- cat abnormal expression than in patients with E - cad or γ- cat normal expression （ median, 16 vs 35 months, P 〈 0.01 ;20 vs 32 months, P 〈 0. 05 ）. While using the proportional hazards regression model （ Cox model ）, E - cad could serve as an independent prognosis factor. There was a positive relationship between the expression of E -cad and γ-cat （r = 0. 416,P = 0. 007 ）. When E - cad and γ-cat were analyzed as one group, lymph node metastasis rate was lower （P 〈0.01 ）and survival time was longer（ median, 45 vs 20 months, P 〈0.01 ） in patients with E -cad and γ-cat normal expression than in patients with only one normal expression of these two proteins. Conclusion ： E - cad and γ- cat play important roles in development and progression of NSCLC. E - cad and γ- cat may be useful to determine prognosis and clinical strategies. Moreover, when E- cad and γ-cat were examined together, the accuracy of predicting lymph node metastasis and prognosis of NSCLC could be increased.

关 键 词：非小细胞肺癌 E-CAD γ-cat 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]