大肠腺瘤及其癌变微卫星不稳定性与细胞增殖的研究  

The study of the relationship between microsatellite instability in adenoma and adenoma with malignant changes of large bowel and proliferation

作　　者：周琪[1] 阎晓初[2] 梁后杰[2] 周进明[3] 吴峰[2] 柳凤轩[2] 

机构地区：[1]重庆市涪陵中心医院消化内科,重庆408000 [2]第三军医大学西南医院病理研究所,重庆400038 [3]第三军医大学西南医院肿瘤科,重庆400038

出　　处：《现代肿瘤医学》2007年第11期1622-1625,共4页Journal of Modern Oncology

基　　金：重庆市卫生局青年人才基金资助(2004097)

摘　　要：目的:探讨微卫星不稳定性(MSI)在大肠腺瘤癌发生中的意义及其对大肠腺瘤、腺瘤癌变组织细胞增殖活性的影响。方法:采用聚合酶链反应-简单序列长度多态性(PCR-SSLP)及免疫组化EnvisionTM方法,对36例大肠腺瘤及12例腺瘤癌变的MSI和增殖细胞核抗原(PCNA和Ki-67)表达进行检测。结果:大肠腺瘤及其癌变组织MSI总阳性率18.75%(9/48),其中,两个位点以上7例阳性,阳性率为15.58%(7/48);一个位点2例阳性。MSI阳性大肠腺瘤及其癌变的PCNA和Ki-67标记指数显著低于MSI阴性者(P<0.05)。结论:MSI可能是结直肠癌发生的早期分子改变,MSI阳性结直肠癌细胞增殖活性较低。To explore the role of microsatellite instability （MSI） in colorectal carcinogensis and the influence on proliferation activity in colorectal adenomas and adenomas with malignant changes. Methods： PCR - SSLP and immunohistochemistry methods were used to detect MSI and expressions of PCNA and Ki- 67 in 36 cases of colorectal adenomas and 12 cases of adenomag with malignant changes. Results ： The total positive rate of MSI was 18.75% （9/48） in colorectal adenomas and adenomas with malignant changes , the MSI positive cases more than two loci were 7 cases, its positive rate was 15.58% （7/48）. The PCNA and Ki - 67 labelling index of MSI positive tumors were significantly lower than that of MSI negative tumors （ P 〈 0.05 ）. Conclusion： MSI may be an early molecular event and change in colorectal carcinogenesis . The MSI positive tumors have low oroliferation activity.

关 键 词：结肠直肠肿瘤 微卫星不稳定性 细胞增殖 

分 类 号：R735.34[医药卫生—肿瘤]