乳腺癌FHIT基因微卫星不稳定性及其蛋白表达的研究  

Study on the microsatellite instability and protein expression of FHIT gene in breast cancer

作　　者：冉贵萍[1] 朱岷[1] 杨文秀[1] 欧阳炜炜[2] 

机构地区：[1]贵阳医学院,贵州贵阳550004 [2]贵阳医学院附属医院

出　　处：《山东医药》2007年第27期7-9,共3页Shandong Medical Journal

摘　　要：目的分析乳腺癌脆性三联组氨酸基因(FHIT)微卫星不稳定性发生情况及与FHIT蛋白表达缺失的关系,以探讨乳腺癌发生的机制。方法选取FHIT基因的D3S1234、D3S1300位点,采用PCR、聚丙烯酰胺凝胶电泳、银染法,对66例乳腺癌患者肿瘤组织进行微卫星不稳定性(MSI)检测,用免疫组化方法检测FHIT蛋白的表达情况。结果30.3%(20/66)的乳腺癌组织至少在一个位点出现MSI;59.1%(39/66)的肿瘤组织发生FHIT蛋白表达缺失或明显下降,17例MSI(+)病例发生FHIT蛋白低水平表达或缺失,MSI(+)与FHIT蛋白表达缺失有相关性(P<0.05)。FHIT蛋白表达缺失与肿瘤组织学分级有相关性(P<0.05)。结论FHIT基因位点的微卫星不稳定性是FHIT蛋白表达下调的可能的分子机制之一,FHIT基因在乳腺癌的发生发展中起重要作用。[ Objective] To analyze the incidence of microsatellite instability （MSI） of FHIT gene in breast cancer and its association with the loss of FHIT protein expression. And to find the possible molecular mechanism of breast tumorigenesis. [ Methods] With PCR-denaturing polyacrylamide gel electrophoresis and silver-staining, 66 specimens of breast cancer were examined for MSI at D3S1234, D3S1300 of FHIT gene. The expression of FHIT protein was analyzed by using immunohistochemical method. [ Results ] 30.3% （20/66） samples of breast cancer were found MSI-positive at least at one locus. Lost or obviously decreased FHIT protein expression was shown in 59.1% （39/66） cases. 17 tumors with M SI （ ＋ ） showed complete loss or obvious decrease of FHIT protein expression. There was significant correlation between MSI of FHIT gene and loss of FHIT protein expression （ P 〈 0.05 ）. There was significant correlation between loss of FHIT protein expression and histological grade （ P 〈 0.05 ）. [ Conclusion] MSI of FHIT gene might be one of possible mechanism which inactivate FHIT gene and suppress the expression of FHIT protein. FHIT gene plays an important role in the development and progression of breast cancer.

关 键 词：FHIT基因 微卫星不稳定性 FHIT蛋白 乳腺肿瘤 

分 类 号：R737.9[医药卫生—肿瘤]