DLC表面特异性吸附血浆蛋白的机理研究  

Mechanism of Formation of Distinctive Serum Proteins Adsorbability on Diamond Like Carbon Film

作　　者：李伯刚[1] 殷杰[1] 那娟娟[1] 尹光福[1] 

机构地区：[1]四川大学材料科学与工程学院,四川成都610065

出　　处：《稀有金属材料与工程》2007年第A02期61-64,共4页Rare Metal Materials and Engineering

基　　金：国家自然科学基金(39870232)资助

摘　　要：为探讨类金刚石薄膜(DLC)与金刚石薄膜(DF)、石墨具有不同血浆蛋白吸附特性的内在原因,进行了人血白蛋白(HSA)和纤维蛋白原(HFG)在3种碳素材料表面吸附前后的红外光谱分析。结果显示:通过-NH基,在DLC与HAS之间及DF、石墨与HFG之间有氢键形成。有力支持并合理解释了DLC具有较高的HSA吸附活性、DF和石墨则优先吸附HFG的前期研究结论。To clarify the reason causing difference of serum proteins adsorbability on different carbonaceous materials, FT-IR-ATR spectra of human serum albumin （HSA） and human serum fibrinogen （HFG） before and after adsorbing on diamond like carbon film （DLC）, diamond film （DF） and graphite were analyzed. It has been shown that there are hydrogen bond because of-NH on the interfaces of HSA-DLC, HFG-DF and HFG-graphite. Based on the results, earlier research conclusion that the adsorbability of HSA on DLC is higher than that on DF and graphite, but on DF and graphite the adsorption of HFG takes precedence can be explained rationally.

关 键 词：类金刚石薄膜 血液相容性 蛋白吸附 氢键 

分 类 号：R318.08[医药卫生—生物医学工程] R542.5[医药卫生—基础医学]