P-gp、GST-π、TopoII在乳腺癌中的表达及其意义  

The Expression of P-gp, GST-π and Topo II in breast carcinoma and its clinical significance

作　　者：李占文[1] 杨华锋[1] 胡云锵[1] 刘纯[1] 李振平[1] 范风风[1] 

机构地区：[1]宁波市妇女儿童医院乳腺外科,315012

出　　处：《浙江医学》2007年第9期919-921,共3页Zhejiang Medical Journal

摘　　要：目的探讨P-糖蛋白(P-gp)、谷胱甘肽S转移酶π(GST-π)及DNA拓扑异构酶II(TopoII)在乳腺癌组织中的表达及其意义。方法采用免疫组化SP法检测96例术前未行化疗的乳腺癌组织中P-gp、GST-π、TopoII以及雌激素受体(ER)、孕激素受体(PR)和人表皮生长因子受体2(HER2)的表达。结果P-gp、GST-π、TopoII的阳性表达率分别为41.7%、42.7%、69.8%。P-gp的阳性表达与肿瘤大小、分期以及腋淋巴结转移均有关(均P<0.05);在组织学分级II、III级者、ER/PR阴性者和HER2阳性者中P-gp的阳性表达率有增高趋势,但无统计学意义(均P>0.05)。GST-π的表达在腋淋巴结阳性者和组织学分级II、III者中有增高趋势。TopoII的阳性表达在组织学分级I级较II、III级有增高趋势,但无统计学意义(P>0.05)。P-gp、GST-π、TopoII存在共表达情况。结论乳腺癌组织的原发多药耐药与P-gp、GST-π、TopoII的表达有关,化疗前对它们进行检测可对化疗药物的选择及预后判断提供参考依据。Objective To investigate the expression of P-glycoprotein （P-gp）,glutathione-s-transferases π （GST-π） and topoisomerase Ⅱ （TopoⅡ） in breast cancer and its clinical significance. Methods Tissue specimens were collected from 96 cases of breast cancer without preoperative chemotherapy. Expression of P-gp, GST-π and TopoⅡ in was examined by immunohistoehemistry. Results The positive expression rate of P-gp, GST-π and TopoⅡ was 41.7%, 42.7% and 69.8% in breast cancer tissues respectively. There was significant relationship between the P-gp and tumor size, TNM stage and lymphnode metastasis （P〈0.05）. The positive expression rate of P-gp was increased in cases with lower differentiation degree, negative expression of ER/PR and positive expression of HER2 but there were no significant differences （P〉0.05）. The positive expression of GST-π was increased in cases with lymphnode metastasis and lower differentiation degree, but no significant differences （P〉0.05）. The positive expression of TopoⅡ was increased in cases with higher differentiation degree but no significant differences （P〉0.05）. In some cases P-gp, GST-π and TopoⅡ co-expressed in breast cancer tissues. Conclusion Detec.ting the expressian of P-gp, GST-π and TopoⅡ before chemotherapy may provide evidence for drug selection and disease prognosis.

关 键 词：乳腺肿瘤 多药耐药性 P-糖蛋白 谷胱甘肽S转移酶Π 拓扑异构酶 

分 类 号：R737.9[医药卫生—肿瘤]