猪流行性腹泻病毒纤突蛋白基因克隆与序列分析  

Cloning and Characterization Analysis of Spike Protein Gene of Porcine epidemic diarrhea virus

作　　者：李建强[1,2] 柳纪省[2] 兰喜[2] 程杰[1] 杨敏[2] 吴润[1] 殷相平[2] 李宝玉[2] 李学瑞[2] 杨彬[2] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,兰州730046

出　　处：《农业生物技术学报》2007年第4期562-566,共5页Journal of Agricultural Biotechnology

基　　金：国家重点基础研究发展规划(973)项目(No.2004CCA00500)资助

摘　　要：对猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)DX株纤突蛋白基因进行了克隆与测序。结果表明,该基因核苷酸序列长为4152bp,推导氨基酸序列为1383aa,与比较毒株氨基酸同源性都在90%以上。系统发生树分析结果表明,比较毒株可分为3个基因群,而且每个群的毒株均来自同一个国家。利用生物软件分析结果表明PEDV发生微小的变异,尤其在韩国变异明显,而DX株与中国JS-2004-2株亲缘关系很近。The spike protein gene of Porcine epidemic diarrhea virus （PEDV） DX strain was cloned and sequenced. The results showed that the nucleotide sequence of the gene was 4 152 bp in length and encoding a protein of 1 383 aa, the homology of amino acids between it and other PEDV strains exceeded 90%. The results ofphylogenetic tree analysis showed that all the reference PEDV isolates could be divided into 3 genetic groups and each of them in the same group came from the same country. Bio-sofl analysis showed that PEDV mutated slowly, especially in Korea. And DX strain and JS-2004-2 strain in China had a closed relationship.

关 键 词：猪源冠状病毒 猪流行性腹泻病毒 纤突蛋白基因 克隆 特征分析 

分 类 号：S188[农业科学—农业基础科学]