高迁移率族蛋白1对多形核白细胞黏附和游出肺毛细血管内皮细胞的影响及其机制  

Effect and mechanism of high mobility group box 1 protein on adhesion to pulmonary capillary endothelial cells and transendothelial mig ration of polymorphonuclear leucocytes

作　　者：杨智勇[1] 陶京[1] 周峰[1] 熊炯忻 吴河水[1] 王春友[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胰腺外科,湖北省武汉市430022

出　　处：《世界华人消化杂志》2007年第22期2403-2407,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30600593~~

摘　　要：目的:探讨高迁移率族蛋白1(HMGB1)对多形核白细胞(PMN)黏附和游出肺毛细血管内皮细胞(PCEC)的影响机制.方法:分离培养大鼠PMN和PCEC,分别加入0,10,100,1000和10 000μg/L浓度的rHMGB1与PCEC单层培养.记录PMN黏附率.在Bovden小室迁移模型中加入上述浓度的rHMGB1,观察PMN穿过PCEC的迁移率.将PMN与100μg/L的rHMGB1孵育,流式细胞仪检测其表面CD11a/CD18和CD11b/CD18的表达率.结果:随着rHMGB1浓度的增加,PMN的粘附率(2.5%±0.5%,5.1%±0.9%,10.7%±1.7%,14.6%±2.6%,25.4%±4.3%)和穿过PCEC的游出率(0%,1.1%±013%,613%±1.2%,12.4%±2.7%,14.2%±3.1%)逐渐增加,但对向下室的迁移率无影响,州MGB1可明显增加PMN表面CD11a/CD18和CD11b/CD18表达的阳性率(均P<0.05)。结论:HMGB1通过上调CD11a/CD18和CD11b/CD18的表达增加PMN对PCEC的黏附率和游出率。AIM： To observe the effect of high mobility group box 1 protein （HMGB1） on adhesion to pulmonary capillary endothelial cells （PCECs） and transendothelial migration of polymorphonuclear leucocytes （PMNs）, and to investigate the mechanism involved. METHODS： PMNs and PCECs of rats were isolated and cultured. PCEC monolayers were cultured with different concentrations of rHMGB, 10, 10, 10G 1000 or 10 000 μg/L. The adhesion rate of PMNs was then detected. A migration model of a boyden chamber was used to evaluate chemotaxis of HMGB1 to PMNs. Transendothelial migration was also investigated. PMNs were cultured with 100μg/L rHMGB1 and the expression of CD11a/CD18 and CD11b/CD18 was detected by flow cytometry. RESULTS： With increasing rHMGB1 concentration, adhesion and transendothelial migration rates of PMNs gradually increased （adhesion rate, 2.5 ± 0.5%, 5.1±0.9%, 10.7 ±1.7%, 14.6 ±2.6%, and 25.4 ± 4.3%; transendothelial migration rate, 0%, 1.1% ± 0.3%, 6.3 ± 1.2%, 12.4 ± 2.7%, and 14.2 ± 3.1%）. rHMGB1 could increase CD11a/CD18 and CD11b/CD18 expression of PMNs （both P 〈 0.05）. CONCLUSION： HMGB1 is not a chemotactic factor for PMNs but it can promote their adhesion and transendothelial migration by increas- ing expression of CD11a/CD18 and CD11b/ CD18 on the surface of PMNs.

关 键 词：高迁移率族蛋白1 多形核白细胞 肺毛细血管内皮细胞 黏附 游出 CD11/CD18 流式细胞仪 

分 类 号：R576[医药卫生—消化系统] R563[医药卫生—内科学]