海水真菌定量分析方法的初步研究  

Preliminary study on the quantitative analysis of marine fungi by plate count method

作　　者：朱红[1] 许忠能[1] 林小涛[1] 孙军[1] 

机构地区：[1]暨南大学水生生物研究中心,广州510632

出　　处：《生态科学》2007年第2期155-158,共4页Ecological Science

基　　金：广东省自然科学基金资助项目(000719)

摘　　要：为定量分析海水真菌,分别采用不同的采样方法采样;并采用5种培养基,每种培养基分别用4种不同的处理方式,共20种不同处理组合,筛选出适合定量分析海水中真菌的平板计数方案。结果表明,不同的采样方法下,真菌在同一培养基上的生长无显著性差异。在同一海水水样中,马铃薯培养基和马铃薯葡萄糖琼脂培养基用海水配制时几乎没有真菌生长;麦芽汁培养基在不同的处理条件下真菌略有生长,且各种处理间无明显差别,其种类主要是霉菌;察氏培养基和酵母菌浸出粉胨葡萄糖琼脂培养基在不同处理条件下真菌长势差别较大,种类主要是酵母菌。通过对20种培养组合的比较,选择酵母浸出粉胨葡萄糖琼脂培养基,用海水来配制培养基并用海水稀释样品的处理,可以培养出最大数量的海洋真菌。In order to develop a method for counting the number of seawater fungi using culture medium flat, the samples were collected by different sampling methods with sterile syringes, organic glass hydrophore, and （then） were diluted by two liquids - seawater and freshwater - and incubated by five culture mediums - Potato agar （PA）, Potato dextrose agar （PDA）, Malt extract media （MA）, Czapek-dox agar （CA） and Yeast extract peptone dextrose agar （YPD）, Each medium was blended with seawater and freshwater, respectively. Results showed that 1） the amount of fungi in the same medium had no obvious difference with different sampling method; 2） there were hardly any fungi in PDA and PA mediums blended with seawater; 3） a few mildews developed in MA medium without obvious differences among the disposals of different liquids in sample dilutions or medium blend; and 4） the populations of fungi-mainly yeasts-in YPD and CA mediums showed differences among the different disposals, with the highest fungal loads observed in seawater YPD medium when seawater sample was diluted by freshwater. Thus, it demonstrated that the seawater YPD with sample diluted by freshwater was more suitable for counting fungi in seawater than other treatments in the taresent study.

关 键 词：海洋真菌 平板计数法 定量分析 培养基 

分 类 号：X172[环境科学与工程—环境科学]