HIF-1α及耐药蛋白在胰腺癌中的表达及相关性分析  

Expression and correlation of Hypoxia inducible Factor 1αand drug-resistance-correlated proteins in human pancreatic carcinoma

作　　者：孙晶[1] 曹俊[1] 江弢[1] 张放[1] 黄陈[1] 裘正军[1] 

机构地区：[1]上海交通大学附属第一人民医院普外科,上海市200080

出　　处：《世界华人消化杂志》2007年第23期2503-2506,共4页World Chinese Journal of Digestology

基　　金：上海市卫生局科技发展基金项目;No.024012~~

摘　　要：目的:探讨HIF-1α及耐药相关指标P糖蛋白(P-Gp),凋亡相关Bcl-2家族成员Bcl-X_L及Bax在人胰腺癌组织中表达的相关性.方法:免疫组化法检测手术切除的胰腺癌组织34例中HIF-1α、P-Gp、Bcl-X_L、Bax蛋白表达.结果:34例胰腺癌标本中有26例(76.5%)HIF- 1α,28例(82.4%)P-Gp,30例(88.2%)Bcl-X_L,26例(76.5%)Bax表达阳性.HIF-1α和P-Gp、Bcl-X_L、Bax蛋白表达呈正相关(r=0.471,P= 0.005;r=0.443,P=0.009;r=0.510,P=0.002).结论:在胰腺癌组织中HIF-1α、P-Gp、Bcl- X_L、Bax蛋白存在高表达;HIF-1α与P-Gp、Bcl- X_L、Bax的蛋白表达呈正相关,提示HIF-1α可能通过上调耐药相关蛋白P-Gp、Bcl-X_L及Bax蛋白表达在胰腺癌耐药机制中起重要作用.AIM： To investigate the expression of Hypoxia inducible Factor 1α（HIF-1α） and the drugresistance-correlated indexes P-glycoprotein （P-Gp）, Bcl-XL and Bax, and the relationship between HIF-1α and resistance to chemotherapy in human pancreatic carcinoma. METHODS： Protein expression of HIF-1α, P-Gp, Bcl-XL and Bax in pancreatic tissues from 34 radically resected specimens of pancreatic carcinoma was detected by immunohistochemical staining. RESULTS： The protein expression rates of HIF- 1α, P-Gp, Bcl-XL and Bax in pancreatic carcinoma were 26/34 （76.5%）, 28/34 （82.4%）, 30/34 （88.2%） and 26/34 （76.5%）, respectively. There were positive correlations between the positivity rates of HIF-1α and P-Gp, Bcl-XL and Bax （r = 0.471, P =0.005; r = 0.443, P = 0.009; r = 0.510, P = 0.002） CONCLUSION： HIF-1α, P-Gp, Bcl-XL and Bax were over-expressed in human pancreatic carcinoma tissues. A positive correlation was determined between the expression rates of HIF- 1α and P-Gp, Bcl-XL and Bax. HIF-1α may have a significant role in resistance to chemotherapy in pancreatic carcinoma via up-regulation of the expression of P-Gp, Bcl-XL and Bax.

关 键 词：胰腺癌 缺氧诱导因子-1Α 化疗耐药 多药耐药基因 凋亡 

分 类 号：R735.9[医药卫生—肿瘤]