大田软海绵酸对FL细胞DNA的损伤及凋亡相关蛋白表达的影响  

DNA DAMAGE AND ALTERATION OF EXPRESSION IN APOPTOSIS RELATED PROTEINS INDUCED BY OKADAIC ACID IN FL CELLS

作　　者：邢鸣鸾[1] 楼建林[2] 徐立红[1] 

机构地区：[1]浙江大学医学院生物化学与遗传学系,杭州310058 [2]浙江大学医学院环境医学系,杭州310058

出　　处：《水生生物学报》2007年第5期661-665,共5页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(20137010)资助

摘　　要：本文旨在探讨大田软海绵酸对人羊膜细胞DNA的损伤及凋亡相关蛋白表达的影响。实验用0、20、40、608、0、100 nmol/L OA诱导FL细胞4h后,检测DNA损伤程度的彗星实验表明,OA对FL细胞DNA的损伤随染毒浓度的升高而增加。蛋白免疫印迹法显示凋亡相关蛋白Bcl-2、Bax和p53的表达与染毒浓度呈负相关;用100 nmol/L OA分别诱导2h、4h、8h后发现,三种蛋白的表达与染毒时间也呈负相关。由此可知在OA诱导的FL细胞凋亡中,损伤DNA,降低Bcl-2蛋白的表达可能参与了凋亡的部分作用,而Bax和p53蛋白则可能与OA诱导的细胞增殖有关。The objective of this work was to evaluate deoxyribonucleic acid （DNA） damage and expression of Bcl-2, Bax, p53 proteins in okadaic acid-treated human amniotic FL cells. Cells were cultured for 4 h with 0, 20, 40, 60, 80 and 100 nmol okadaic acid （OA）, or 100 nmol OA for periods of 2, 4 and 8 h. Comet assay results indicated that DNA damage was a dosedependent increase and western blot analysis showed that the expression of Bcl-2, Bax, p53 proteins was time- and dose-depen- dent decrease. Therefore, it suggested that DNA damage and Bcl-2 might play a partial role in OA-induced FL cell apoptosis, and on the contrary, Bax and p53 were related to the proliferation of FL cell.

关 键 词：大田软海绵酸 DNA损伤 BCL-2 BAX P53 

分 类 号：X173[环境科学与工程—环境科学]