亚低温离体大鼠肝细胞悬浮培养后的功能  

The study of integrity and function of hepatocytes during extended suspension culture at 30℃

作　　者：叶启发[1] 袁金忠[1] 明英姿[1] 熊力[1] 牛英[1] 

机构地区：[1]中南大学湘雅三医院湘雅移植医学研究院,长沙410013

出　　处：《中华实验外科杂志》2007年第9期1067-1069,共3页Chinese Journal of Experimental Surgery

基　　金：湖南省自然科学基金(04JJ6017);2005年国家卫生部重大课题资助项目(98040362)

摘　　要：目的观察亚低温条件下离体大鼠肝细胞悬浮培养后的完整性和功能。方法离体肝细胞悬浮于添加有葡萄糖和牛血清白蛋白(BSA)的碳酸氢盐缓冲介质内30℃下孵育48 h,期间及其后测定细胞的完整性和功能指标。结果30℃条件下离体0、24及48 h内白蛋白合成及Ⅰ相药物生物转化能力差异无统计学意义(P>0.05),流式细胞仪活细胞计数和台盼蓝清除率显示细胞完整性差异无统计学意义(P>0.05)。结论肝细胞混悬液内的离体肝细胞在亚低温条件下能够保持较高活力,为制备应用于生物人工肝的离体肝细胞混悬液提供了可行性。Objective To study the integrity and function of hepatocytes during extended suspension culture at mild hypothermia. Methods Isolated hepatocytes were suspended a bicarbonate buffered saline medium supplemented with glucose and bovine serum albumin（BSA） for 48 hours at 30 ℃ ,during and after which, the integrity and function of hepatocytes were assessed. Results Under the up-mentioned condition there was no significant decline in cell viability, albumin synthesis and phase I drug detoxification activity of these cells during 48 h post isolation. Conclusion These observation demonstrate the robust nature of mildly hypothermia isolated hepatocytes in suspension and encourage further studies re-exaruing the feasibility of using this cell preparation in bioartificial liver.

关 键 词：生物人工肝 亚低温 白蛋白合成 离体肝细胞 悬浮培养 

分 类 号：R575[医药卫生—消化系统]