2709碱性蛋白酶水解脱铬革屑的研究  

Hydrolysis of Dechromed Chrome Shavings with 2709 Alkalic Protease

作　　者：陈秀金[1] 侯颖[1] 张敏[1] 朱文学[1] 汤克勇[2] 

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471003 [2]郑州大学材料科学与工程学院,河南郑州450052

出　　处：《中国皮革》2007年第19期47-50,共4页China Leather

基　　金：国家自然科学基金(50473021);河南科技大学校基金项目(2004QN026)

摘　　要：研究了采用2709碱性蛋白酶水解脱铬革屑制备胶原水解产物时,影响胶原水解率的各种因素。研究表明,最佳水解条件为:酶量0.12%,pH10.0,温度50℃,时间40min。在此条件下,胶原的平均水解率为62.9%。同时测定了最佳水解条件下所得胶原水解产物的理化特性。2709 alkalic protease was used to hydrolyse dechromed chrome shavings, and the factors that may affect the collagen hydrolysis ratio, including the amount of 2709 alkalic protease, temperature, pH and time of hydrolyzing reaction, were studied. The optional hydrolyzing conditions were as follows： alkalic protease 0.12%, pH 10.0, 50℃, 40min. Under such conditions, the average hydrolysis ratio of collagen was 62.87 %, and the physical and chemical properties of collagen hydrolysate were also studied.

关 键 词：2709碱性蛋白酶 脱铬革屑 胶原水解产物 理化特性 

分 类 号：TS54[轻工技术与工程—皮革化学与工程]