PTEN与预后相关因子在乳腺癌组织芯片中的相关性  

Microarray analysis of the relationship between PTEN and prognostic factors in primary breast cancer tissues

作　　者：周隽[1] 王军臣[2] 卢婉平[2] 符雪莲[2] 鲁昌立[2] 于静[2] 张惠箴[1] 

机构地区：[1]上海市交通大学附属第六人民医院病理科,上海200233 [2]上海市同济大学附属东方医院病理科,上海200120

出　　处：《肿瘤》2007年第9期723-726,共4页Tumor

基　　金：上海市浦东新区社会发展局卫生科技发展专项基金项目(编号:PW20046-3)

摘　　要：目的:构建乳腺癌组织芯片,研究PTEN与乳腺癌预后相关因子ER、PR、c-erbB-2、Ki67在原发性乳腺癌中的蛋白表达及其相关性。方法:制作乳腺癌组织芯片,用免疫组织化学法检测芯片上165例乳腺癌组织中PTEN、ER、PR、c-erb B-2和Ki67蛋白的表达。结果:乳腺癌组织中PTEN的高表达例数为66/163(40.5%),ER、PR、c-erb B-2和Ki67阳性率分别为51.5%、35.6%、34.4%、44.8%。PTEN阳性表达随腋淋巴结转移数、组织学分级和TNM分期的增高而降低,呈负相关性P<0.05),与肿瘤的大小和发生年龄无明显相关。乳腺癌中PTEN与ER、PR蛋白表达呈显著正相关,与c-erb B-2、Ki67蛋白表达呈负相关。结论:PTEN蛋白低表达及表达缺失与性激素失调及乳腺肿瘤细胞的生长、预后之间存在一定的联系,PTEN可作为判断乳腺癌恶性程度的潜在标志物。同时检测PTEN、ER、PR、c-erb B-2和Ki67对乳腺癌的辅助治疗具有重要意义及实用价值。Objective ： To construct tissue microarray of breast carcinoma and to investigate the association of hepatocyte-specific phosphatase and tensin homolog （PTEN） protein expression with prognostic factors such as estrogen receptor （ER）, progesterone receptor （PR）, c-erb B-2, and Ki-67 in primary breast carcinoma. Methods：Tissue microarray of breast carcinoma was constructed. Immunohistochemical staining was used to detect the expression of PTEN, ER, PR, c-erb B-2, and Ki-67 in breast carcinoma tissues. Results： PTEN was highly expressed in 66 out of 163 breast carcinoma tissues （40.5%）. The positive rate of ER, PR, c-erb B-2, and Ki-67 was 51.5%, 35.6% , 34.4% , and 44.8% , respectively. The positive expression level of PTEN decreased with increase in auxiliary lymph nodes metastasis, histological grading, and TNM staging. They had negative correlations （ P 〈 0.05 ）. The positive expression level of PTEN had no evident correlation with tumor size and onset age. PTEN had positive correlation with the expressions of ER and PR protein （P 〈0.01 ）, and had negative correlation with the expressions of c-erb B-2 and Ki-67. Conclusion：There exists some relationships between low or loss of PTEN expression and the imbalanced expression of ER and PR and the growth and prognosis of breast carcinoma. PTEN may be considered as a potential indicator to judge the malignant degree of breast carcinoma. Combined detection of PTEN, ER, PR, c-erb B-2 and Ki-67 have significant importance and practical value for adjuvant therapy of breast carcinoma.

关 键 词：乳腺肿瘤 蛋白质阵列分析 PTEN ER PR C-ERB B-2 KI67 

分 类 号：R737.9[医药卫生—肿瘤]