食管鳞癌中Ezrin和CD44-v6的表达及其临床意义  

Expression of ezrin and CD44-v6 in human esophageal squamous cell carcinoma and its clinical significance

作　　者：柴立勋[1] 孙克林[1] 郭黎平[2] 张宏图[3] 陆士新[2] 

机构地区：[1]中国医学科学院肿瘤医院胸外科,北京100021 [2]中国医学科学院肿瘤医院病因室,北京100021 [3]中国医学科学院肿瘤医院病理科,北京100021

出　　处：《中华肿瘤杂志》2007年第9期685-688,共4页Chinese Journal of Oncology

摘　　要：目的探讨ezrin和CD44-v6在食管鳞癌中的表达及其与淋巴结转移,病理学分级等的关系。方法应用组织芯片技术进行免疫组化S-P法染色,检测71例食管鳞癌患者癌组织标本及癌旁正常上皮中ezrin和CC44-v6的表达情况,并结合临床相关因素进行统计学分析。结果71例癌旁正常上皮中,ezrin表达阳性33例,CD44-v15表达阳性18例;71例食管鳞癌组织中,ezrin表达阳性64例,CD44-v6表达阳性58例。Ezrin的表达与淋巴结转移关系密切,有淋巴结转移组ezrin强阳性率明显高于无淋巴结转移组(P<0.05);CD44-v6的表达与肿瘤组织的病理学分级、淋巴结转移关系密切,在淋巴结转移组CD44-v6表达阳性率显著高于无淋巴结转移组(P<0.05)。CD44-v6阳性表达组的ezrin表达阳性率显著高于CD44-v6阴性表达组(P<0.05)。Ezrin与CD44-v6同时表达的48例患者中,淋巴结转移率高达60.0%,而ezrin与CD44-v6同时不表达的6例患者中,均无淋巴结转移。结论联合检测ezrin和CD44-v6的表达有助于综合判断食管鳞癌的恶性程度和转移潜能,ezrin有望成为判断肿瘤预后的新指标。Objective To evaluate the expression of ezrin and CIM4-v6 in esophageal squamous cell carcinoma, and to evaluate its relationship with lymph node metastasis （LNM） and histological grading. Methods The expression of ezrin and CIM4-v6 in 71 patients with esophageal squamous cell carcinoma was studied using immunohistochemical （SP） method. The correlation of their expression with relevant clinical data was statistically analyzed. Results In normal esophageal squamous epithelia, the expression of ezrin was found in 33 cases among 71 cases and the expression of CIM4-v6 in 18 cases among 71 cases. In esophageal squamous cell carcinoma, the expression of ezrin was found in 64 cases among 71 cases and CIM4-v6 in 58 cases among 71 cases. The expression of ezrin was closely related to LNM. The positive rate of ezrin expression in LNM cases was significantly higher than that in cases without LNM. The expression of CIM4-v6 had a close relation to tumor differentiation and LNM. The positive rate of CIM4-v6 expression in LNM cases was significantly higher than that in patients without LNM. The expression of ezrin in CIM4-v6 positive cases was significantly higher than that of CIM4-v6 negative cases. The LNM rate was 60.0% in 48 patients with positive expression of both ezrin and CIM4-v6, while none of lymph node metastasis was found in the 6 patients with both negative. Conclusion The test of CIM4-v6 and ezrin expression may have significant prognostic value for assessing the degree of malignancy and potential LNM probability of ESCC. Ezrin may become a new target in evaluation of tumor prognosis.

关 键 词：食管肿瘤 鳞癌 EZRIN CD44-V6 组织芯片 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]