膜型/可溶型MHCⅠ类链相关分子A及其受体NKG2D在骨肉瘤中的表达及其意义  

Expression of membrane/soluble MHC class I chain-related molecule A and NKG2D receptor in osteosarcoma and its clinical significance

作　　者：肖萍[1] 薛玲[1] 车丽洪[1] 吴惠茜[1] 乔慧[1] 

机构地区：[1]中山大学附属第一医院病理科,广州510080

出　　处：《中华病理学杂志》2007年第9期596-599,共4页Chinese Journal of Pathology

基　　金：国家自然科学基金(30300354)

摘　　要：目的探讨膜型/可溶型 MHC Ⅰ类链相关分子 A(MICA)及其受体 NKG2D 在骨肉瘤中的表达及其意义。方法采用免疫组织化学(LSAB法)检测膜型 MICA(mMICA)在43例骨肉瘤组织中的表达;用流式细胞仪检测上述病例中的16例患者外周血淋巴细胞 NKG2D 的表达;用 ELISA 法检测患者血清中可溶型 MICA(sMICA)的水平。结果骨肉瘤细胞广泛表达 mMICA(37/43),外周血淋巴细胞 NKG2D 的表达普遍下降,两者的表达水平均与骨肉瘤的分化呈正相关(P<0.05),与临床分期呈负相关(P<0.05)。检测16例骨肉瘤患者血清中 sMICA 含量,其中5例升高,且与骨肉瘤的远处转移及临床分期呈正相关(P<0.05)。结论 (1)骨肉瘤细胞表达 mMICA,外周血淋巴细胞NKG2D 的表达以及血清中 sMICA 的含量与骨肉瘤的分化及临床分期相关,因此可望作为骨肉瘤病理诊断和估计预后的生物学指标。(2)MICA-NKG2D 介导的免疫监视障碍可能促进了骨肉瘤的进展。Objective To study the expression of membrane MICA （mMICA）, soluble MICA （sMICA） and NKG2D receptor in cases of osteosarcoma and to analyze its clinical significance, Methods Expression of mMICA in osteosarcoma tissue of 43 cases was detected with immunohistochemistry. Expression of NKG2D in peripheral blood lymphocytes of 16 cases was analyzed by flow cytometry. Serum level of soluble MICA （sMICA） was measured by ELISA, Results mMICA was widely expressed in osteosarcoma tissue （37/43）. Expression of NKG2D in peripheral blood lymphocytes was significantly decreased. High levels of mMICA and NKG2D expression were associated with better differentiation and earlier tumor stage of osteosarcoma （ P 〈 0. 05 ）. A significant increase in serum level of sMICA was demonstrated in patients with metastasis and advanced tumor. Conclusions The mMICA expression in tumor tissue, NKG2D expression in peripheral lymphocytes and serum sMICA level correlate with the differentiation and stage of osteosarcoma. These parameters may thus represent potential diagnostic and prognostic markers in patients with osteosarcoma. Manipulation of the MICA-NKG2D pathway may become a target of immunotherapy for osteosarcoma.

关 键 词：骨肉瘤 基因 MHC I类 

分 类 号：R738.1[医药卫生—肿瘤]