大鼠烫伤后创面渗液中细胞因子的变化  

Dynamic changes in cytokines in wound exudates from superficial Ⅱ° burns in rats

作　　者：吴建和[1] 童善庆[1] 朱莉[1] 胡宝瑜[1] 张希衡[1] 陆德源[1] 密磊 王文奎[2] 冯世杰[2] 仇刚[2] 许伟石[2] 史济湘[2] 

机构地区：[1]上海第二医科大学微生物学教研室,200025 [2]上海第二医科大学附属瑞金医院

出　　处：《中华整形烧伤外科杂志》1997年第1期25-29,共5页

摘　　要：以大鼠为模型,探讨浅Ⅱ度烫伤后不同时期局部渗液中细胞因子的动态变化。120只大鼠分两组。Sb 组:皮下包埋海绵后再造成10%浅Ⅱ度烫伤;S 组:单埋海绵对照。于伤后6,24小时,3,7,10和14天检测海绵渗液中 IL-1、IL-6和肿瘤坏死因子(TNF)的活性。结果三种细胞因子活性的升降规律相似。在致伤72小时内一致表现为短暂骤升和回落,72小时后又持续上升。IL-6于6小时达高峰;IL-1与TNFα于24小时达峰值。早期的细胞因子可能由局部细胞应激产生;后期主要产自单核细胞。当细咆因子活性处于各自峰值时,两组间有较明显差异。其中早期水平 S>Sb,可能的原因:①组织受损 Sb 组更严重,致产细胞因子的局部细胞数 S 组>Sb 组;②烫伤改变了局部组织通透性致使细胞因子外渗。后期Sb 组水平持续较高;另外,IL-1与 IL-6活性在 S 组内有显著相关,而在 Sb 组,仅6小时有较明显相关。提示:Sb 组的修复过程更复杂、耗时。We take rats as models to study the dynamic changes in cytokines in wound exudate at different time phases post-burn.120 SD rats were divided into two groups:group 1 with subcutanous im- plantation of sponges only(S),and group 2 rats were inflicted 10% superficial Ⅱ° burns of their total body surface immediatly after the implantation (Sb).The activities of IL-1、IL-6 and TNFα in wound ex- udates within sponges were measured at 6h,1d,3d,7d,10d and 14d after the injuries.The changes in ac- tivity of these cytokines in both groups showed somewhat the same type of fluctuations,ie,temporarly ebb and flow in the early phases,and 72hs later showing a steady increase.The activity of IL-6 reached the peak level in 6hs while IL-1 and TNFα in 24hs.Probably the early peak levels were produced by lo- cal cells responding to the injuries.At each peak level,there was an apparent difference between group S and group Sb(p<0.01).In the early phases,the levels were S>Sb,and later,the reverse.In group S there was a positive correlation between IL-1 and IL-6,whereas in Sb only 6h's exudate shoWed a weak positive correlation.We think the cause of the early differences was:①less number of cytokine secret- ing cells in Sb due to severer injury to local tissues,and② increased capillary permeability releasing cy- tokines from local tissues to the surroundings.The late differences suggest that repair was more compli- cated and time-consuming in Sb.

关 键 词：烫伤 渗出液 肿瘤坏死因子 IL-1 IL-6 

分 类 号：R644[医药卫生—外科学]