T淋巴瘤侵袭转移诱导因子1表达水平与胃癌生物学行为的关系  

Quantification of T lymphoma invasion and metastasis inducing factor 1 mRNA and its correlation with gastric cancer biological behaviours

作　　者：关宝杰[1] 刘力华[1] 张恩颖[1] 续薇[1] 

机构地区：[1]吉林大学第一医院检验科,长春130021

出　　处：《中华检验医学杂志》2007年第9期1029-1032,共4页Chinese Journal of Laboratory Medicine

基　　金：吉林省科技厅自然科学基金(200505209)

摘　　要：目的探讨 T 淋巴瘤侵袭转移诱导因子1(Tiam 1)在胃癌组织、癌旁组织、胃良性病变组织中的表达水平,及其与胃癌转移等生物学行为的关系。方法用实时荧光定量逆转录 PCR(FQ-RT-PCR)方法,对66例胃癌患者的癌组织、癌旁组织及11例胃良性病变患者的组织标本中Tiaml mRNA 进行定量检测。结果 66例胃癌患者的癌组织、癌旁组织中 Tiaml mRNA 表达阳性率分别为92.4%、19.7%,11例胃良性病变患者的组织标本中有1例患者有 Tiaml mRNA 表达,3类组织标本的差异有统计学意义(x^2=78.96,P<0.01);胃癌组织中 Tiaml mRNA 表达量高于癌旁组织,且与患者性别、年龄、肿瘤大小、肿瘤原发部位无关(P>0.05),但随着淋巴结转移的发生、浸润深度加深、分化程度降低、TNM 分期增高,而呈现增高趋势(P<0.05)。结论 FQ-RT-PCR 检测 Tiaml 表达具有较高的敏感性和特异性,Tiaml 与胃癌生物学行为密切相关,有可能成为胃癌转移的基因治疗新靶点。Objective To evaluate the expression quantity of T lymphoma invasion and metastasis inducing factor 1 （ Tiam 1 ） in gastric cancer tissues, tissues around the cancer over 3 cm and tissues of gastric benign lesions, and to analyze the correlation between amount of Tiaml and gastric cancer biological behaviours. Methods Real-time fluorescence quantitive RT-PCR （FQ-RT-PCR）was used to detect the expression level of Tiaml mRNA in 66 cases of gastric cancer tissues,tissues around the cancer over 3 cm and the gastric tissues of 11 cases with benign lesion. Results Positive rate of Tiaml mRNA expression in gastric cancer tissues, tissues around the cancer was 92. 4%, 19. 7%, respectively, and only one case express Tiaml mRNA in 11 cases gastric benign lesion tissues. There was significant difference （ P 〈 0. 01 ） among the three groups of patients. The amount of Tiaml mRNA in gastric cancer tissues was much higher than in the para-cancerous tissues. There was no obvious correlation between the expression level and sex, age of patients, as well as the sizes or primary location of gastric cancer （ P 〉 0. 05 ）, on the other hand, the expression quantity of Tiaml mRNA in gastric cancer was significantly correlated with metastasis into lymph node,the extent of invasion, differentiated degree of histologic type, and the stages of TNM （P 〈 0. 05 ）. Conclusions FQ-RT-PCR is a sensitive and specific assay for quantificating expression of Tiaml. There exists the correlation between Tiaml expression and gastric cancer biological behaviours. Tiaml may become a novel target for gastric cancer＇s gene therapy.

关 键 词：胃肿瘤 肿瘤转移 T淋巴瘤侵袭转移诱导因子1 

分 类 号：R735.2[医药卫生—肿瘤]