黄热病毒与甲型流感病毒及其分型集合检测芯片的初步研制  

Preparation of oligo microarray for detecting and genotyping of yellow fever virus and influenza A virus

作　　者：黄吉城[1] 张海燕[2] 马文丽[2] 郭波旋[1] 商涛[2] 相大鹏[1] 郑夔[1] 师永霞[1] 洪烨[1] 幸芦琴[1] 李小波[1] 胡龙飞[1] 钟玉清[1] 

机构地区：[1]广东出入境检验检疫局检验检疫技术中心,广州510623 [2]南方医科大学基因工程研究所

出　　处：《中国卫生检验杂志》2007年第10期1732-1734,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家质量监督检验检疫总局科技计划资助项目(2006IK168)

摘　　要：目的:研制黄热病毒与流感病毒及其分型集合检测芯片。方法:设计并合成60mer寡核苷酸(Oligo)探针用于制备检测芯片。提取黄热病、甲型流感病毒核酸,以限制性显示技术进行扩增标记,完成杂交后对芯片进行扫描和数据分析。结果:Oligo探针与相应的荧光标记样本杂交后,大部分能检测出阳性荧光信号,而空白对照和阴性对照均为阴性信号。结论:寡核苷酸芯片技术可应用于多种病毒的检测及分型,从而为多种病毒感染的早期鉴别诊断提供科学依据。Objective：To prepare oligo microarray for detecting and genotyping of yellow fever virus（YFV） and influenza A virus.Methods：The 60-mer oligo-nucleotide probes were designed and synthesized and oligo microarray was prepared by micro-griding.The nucleic acid of influenza A virus and yellow fever virus was extracted with QIAGEN RNA extraction kit,then amplified and labeled by restriction display technology.After hybridization,microarrays were scanned and analyzed.Results：Most of the corresponding oligonucleotide probes were detected to have positive signals.Meanwhile,the negative and blank controls had negative signals.Conclusion：The oligo microarray established by this study can be applied in detection and genotyping of multi-viruses,thus it will be able to provide scientific evidences for clinical differential diagnosis of diseases caused by multi-viruses.

关 键 词：黄热病毒 甲型流感病毒 检测芯片 

分 类 号：R394[医药卫生—医学遗传学]