血管生成素Ⅱ在低压性缺氧成年大鼠视网膜的表达  

Angiopoietin Ⅱ in retina of hypoxic adult rats

作　　者：张勇[1] 王志斌[1] 周霞[1] 许玲[1] 芦晓磊[1] 黄剑云[1] 

机构地区：[1]郧阳医学院附属太和医院眼科,湖北省十堰市442000

出　　处：《眼科新进展》2007年第10期758-761,共4页Recent Advances in Ophthalmology

摘　　要：目的探讨成年大鼠缺氧后血管生成素Ⅱ（angiopoietinⅡ,AngⅡ）在视网膜中的表达情况。方法成年Wistar大鼠暴露于低氧环境（70~90mL.L-1O2）2h后,分别在3h、24h、3d、7d和14d,通过实时荧光定量逆转录聚合酶链反应、蛋白免疫印迹法检测AngⅡ在视网膜中的表达情况。结果缺氧后3h、24h和3d,成年大鼠视网膜AngⅡmRNA水平与正常对照组相比分别增加120%、50%、30%（P〈0.05）,AngⅡ蛋白质水平与正常对照组相比分别增加了90%、32%、20%,而在7d和14d时其表达较正常对照组差异无显著性。结论缺氧可以使成年大鼠视网膜AngⅡ表达上调。Objective To investigate the expression of angiopoietin Ⅱ （Ang Ⅱ ） in the retina of adult rat following hypobaric hypoxic exposures. Methods Adult Wistar rats were exposed to hypobaric hypoxic environment for 2 hours following which the expression of Aug Ⅱ were observed at 3 hours, 24 hours, 3 days,7 days and 14 days with real time quantitative RT-PCR and Western-blotting,respectively. Results Compared with normal control group, the expression of Aug Ⅱ mRNA were increased by 120% ,50% ,30% and the level of proteins increased by 90% ,32% ,20% ,respectively, following hypobarie hypoxic exposures for 3 hours,24 hours and 3 days（P〈0.05）.There were no significant differences at 7 days and 14 days. Conclusion Hypoxia can upregulate Aug Ⅱ in adult retina

关 键 词：血管生成素Ⅱ 缺氧 视网膜 实时荧光定量逆转录聚合酶链反应 蛋白免疫印迹法 

分 类 号：R774.2[医药卫生—眼科]