Reconstruction of HaSNPV with helicoverpa hormone receptor 3  

作　　者：扈进冬 Shao Honglian Zhang Yubao Fu Qiang Sun Chen Wang Jinxing Zhao Xiaofan 

机构地区：[1]School of Life Sciences, Shandong University, Jinan 250100, P.R. China [2]School of Meclicine, Shangdong University, Jinan 250100, P.R. China

出　　处：《High Technology Letters》2007年第3期327-331,共5页高技术通讯（英文版）

基　　金：Supported by the National Natural Science Foundation of China （30330070）, the High Technology Research and Development Programme of China （2003AA214050） and the Natural Science Foundation of Shandong Province （Z2003D04）. Received on June 13, 2006

摘　　要：In order to develop a more efficient virus for controlling the cotton bollworm Helicoverpa armigera, Helicoverpa hormone receptor 3 （HHR3）, which is involved in the ecdysteroid regulatory pathway, was used to genetically modify wild HaSNPV. HaSNPV-HHR3 budded virus and occlusion body virus were constructed in three steps： preparation of pFastBacHaPhpP10-HHR3 donor plasmid, transposition of HHR3 into the HaBacHZ8 bacmid, and transfection of HzAM1 cells to get HaSNPV-HHR3 virus.HHR3 was proved to be expressed in the HaSNPV-HHR3 virus infected HzAM1 cells by immunoblotting. Results of bioassay indicated that the body weight of the HaSNPV-HHR3 infected larvae was lower than the larvae infected with wild virus and uninfected normal larvae, which suggests that HaSNPV-HHR3 delayed larval growth.

关 键 词：LEPIDOPTERA NOCTUIDAE Helicoverpa armigera Helicoverpa armigera single nucleocapsid nucleopolyhedrovims （HaSNPV） Helicoverpa hormone receptor 3 （HHR3） genetical manipulation BIOASSAY 

分 类 号：Q96[生物学—昆虫学]