BIOASSAY

作品数:160被引量:364H指数:10
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相关作者:林思祖王爱萍杜玲曹光球田文忠更多>>
相关机构:福建农林大学内蒙古师范大学中国科学院中国科学院遗传与发育生物学研究所更多>>
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Digital PCR-free technologies for absolute quantitation of nucleic acids at single-molecule level
《Chinese Chemical Letters》2025年第2期90-98,共9页Xinyi Luo Ke Wang Yingying Xue Xiaobao Cao Jianhua Zhou Jiasi Wang 
supported by the National Key Research and Development Program of China(Nos.2023YFC2307305,2021YFF0703300);the Shenzhen Medical Research Fund(No.B2303003);Shenzhen Research Funding Program(Nos.JCYJ20220818102014028,RCBS20210609104339043);National Natural Science Foundation of China(No.22174167);Guangdong Basic and Applied Basic Research(No.2024A1515011281);Fundamental Research Funds for the Central Universities(No.24qnpy087)from Sun Yat-sen University。
Ultrasensitive detection of nucleic acids is of great significance for precision medicine.Digital polymerase chain reaction(dPCR)is the most sensitive method but requires sophisticated and expensive instruments and a ...
关键词:Digital bioassay Isothermal amplification Nucleic acid detection Digital CRISPR CAS Absolute quantification 
Multiplex analysis platform for evaluation of endocrine disruption of emerging contaminants against human steroid hormone receptors using autobioluminescent yeast bioassay:Application to bisphenols
《Emerging Contaminants》2024年第4期132-144,共13页Yuan Huang Wei Zhang Ruiguo Wang Zhiming Xiao John P.Giesy Liming Wu Xiaoou Su 
supported by the National Key Research and Development Program of China(NO.2023YFD1301505,No.2017YFC1600303);the Agricultural Science and Technology Innovation Program of CAAS(CAAS-ASTIP-IQSTAP-04);Prof.Giesy was supported by the Canada Research Chair program,the 2012"High Level Foreign Experts"(#GDT20143200016)program,funded by the State Administration of Foreign Experts Affairs,the P.R.China to Nanjing University and the Einstein Professor Program of the Chinese Academy of Sciences and a Distinguished Visiting Professorship in the Department of Environmental Sciences,Baylor University in Waco,TX,USA.
Emerging synthetic or derivative chemicals have drawn global concern due to their potential endocrine-disrupting effects,either as agonists or antagonists or through indirect effects on enzymes of signal transduction ...
关键词:teroid hormone receptors YEAST Agonistic activity Antagonistic activity BISPHENOLS Endocrine-disrupting 
Cardioprotective effect of bioassay-guided fractions of Cleome viscosa methanolic extract in streptozotocin-induced diabetic rats
《Asian Pacific Journal of Tropical Biomedicine》2024年第11期486-499,共14页Suresh Yarrappagaari Lavanya Thopireddy Srinivasulu Cheemanapalli Venkata Ramireddy Narala Rajeswara Reddy Saddala 
supported by University Grants Commission-Rajiv Gandhi National Fellowship[F1-17.1/2016-17/RGNF-2015-17-SC-AND 6017/(SAIII/Website)].
Objective:To evaluate the cardioprotective effect of bioassay-guided isolated fractions of Cleome viscosa methanolic extract in streptozotocin-induced diabetic rats.Methods:Total phenolics,flavonoids,and in vitro anti...
关键词:Cleome viscosa L. CARDIOPROTECTION Cardiac markers DOCKING CK-MB GC-MS Diabetes 
Bioinspired bioassay platforms derived from colloidal crystals with topological shapes
《Aggregate》2024年第2期39-60,共22页Huan Wang Hui Zhang Feika Bian Dagan Zhang Hongcheng Gu Bin Kong 
National Natural Science Foundation of China,Grant/Award Numbers:22302231,21902024,82102511,22104154,82102181;Guangdong Basic and Applied Basic Research Foundation,Grant/Award Number:2023A1515011986;Natural Science Foundation of Jiangsu,Grant/Award Numbers:BK20210021,BE20210009,BK20200568;Research Project of Jiangsu Province Health Committee,Grant/Award Number:M2021031;Shenzhen Fundamental Research Program,Grant/Award Number:JCYJ20190808120405672;Futian Healthcare Research Project,Grant/Award Numbers:FTWS2022013,FTWS2023080;Fundamental Research Funds for the Central Universities,Sun Yat-sen University,Grant/Award Number:23qnpy153;Fundamental Research Funds for the Central Universities,Grant/Award Number:2242021R41178;Funding for Clinical Trials from the Affiliated Drum Tower Hospital,Medical School of Nanjing University,Grant/Award Number:2022-LCYJ-PY-05;Shenzhen Medical Academy of Research and Translation Program,Grant/Award Number:A2303017。
Colloidal crystals are materials self-assembled from the colloidal nanoparticles.Due to the ordered microstructure,they exhibit significant optical properties and have shown huge potential in the field of biosensing.B...
关键词:biosensors colloidal crystals HYDROGELS MICROFLUIDICS visual detection 
Barley chitinase genes expression revamp resistance against whitefly (Bemisia Tabaci) in transgenic cotton (Gossypium hirsutum L.)
《Journal of Cotton Research》2024年第1期90-100,共11页BASHIR Samina YAQOOB Amina BASHIR Rohina BUKHARI Shanila SHAHID Naila AZAM Saira BAKHSH Allah HUSNAIN Tayyab SHAHID Ahmad Ali RAO Abdul Qayyum 
Background Chitinase is an enzyme that hydrolyzes chitin,a major component of the exoskeleton of insects,including plant pests like whiteflies.The present study aimed to investigate the expression of chemically synthe...
关键词:CHITINASE Cotton White fly TRANSGENE BIOASSAY 
Discovery and bioassay of disubstituted β-elemene-NO donor conjugates:synergistic enhancement in the treatment of leukemia被引量:2
《Chinese Journal of Natural Medicines》2023年第12期916-926,共11页ZHU Junlong JIANG Xiaoying LUO Xinyu GAO Yuan ZHAO Rui LI Junjie CAI Hong DANG Xiawen YE Xiangyang BAI Renren XIE Tian 
This project was supported by the Natural Science Foundation of Zhejiang province(No.LY20H300004);the National Natural Science Foundation of China(Nos.82073686,81730108,and 81973635);Scientific Research Foundation for Scholars of HZNU(Nos.2021QDL026 and 2019QDL003);the Ministry of Science and Technology of China(High-end foreign experts program,Nos.G20200217005 and G2021017004).
Natural products are essential sources of antitumor drugs.One such molecule,β-elemene,is a potent antitumor compound extracted from Curcuma wenyujin.In the present investigation,a series of novel 13,14-disubstituted ...
关键词:Antitumor activity Chronic myeloid leukemia Β-ELEMENE Nitric oxide donor Structural modification 
Bioassay of Carcinoembryonic Antigens by Organic Field-effect Transistors Based on D-A Type Conjugated Polymer
《Chemical Research in Chinese Universities》2023年第6期877-883,共7页LYU Xiaomeng DUAN Yuchen CHEN Yulan CHENG Shanshan 
supported by the National Natural Science Foundation of China(Nos.22275068,21975178);the Open Project of the State Key Laboratory of Supramolecular Structure and Materials,China.
Biosensors based on organic field-effect transistors(OFETs)are one of the most promising electronic devices for emerging bioanalytical applications.The selection of organic semiconductors(OSCs)is essential to improve ...
关键词:Organic field-effect transistor Conjugated polymer Carcinoembryonic antigen BIOSENSOR SEMICONDUCTOR 
Design,synthesis and bioassay of the emerging photo-responsive fungicides
《Chinese Chemical Letters》2023年第8期223-226,共4页Wen Fu Xinyue Hu Qinglong Yuan Zhiping Xu Jiagao Cheng Zhong Li Xusheng Shao 
This work was financially supported by the Natural Science Foundation of China(Nos.21877039,32072441);National Key Research and Development Program of China(No.2018YFD0200100);Innovation Program of Shanghai Municipal Education Commission(No.2017-01-07-00-02-E00037).
Precise and spatiotemporal control over the pesticide remains to be a challenge.More efficient controlled release systems(CRSs)have been developed to support the precise delivery of active ingredients.Herein,we incorp...
关键词:Phenamacril Controlled release Photoremovable protecting groups SPATIOTEMPORAL Significant difference 
Bioassay-guided isolation of α-Glucosidase inhibitory constituents from Hypericum sampsonii
《Chinese Journal of Natural Medicines》2023年第6期443-453,共11页TAO Linlan XU Shuangyu ZHANG Zizhen LI Yanan YANG Jue GU Wei YI Ping HAO Xiaojiang YUAN Chunmao 
supported by the National Natural Science Foundation of China(No.32270413,U1812403);the Science and Technology Department of Guizhou Province(No.QKH 2020-1Z076);the 13th batch of Outstanding Young Scientific and Technological Talents in Guizhou Province(No.QKHPTRC[2021]5633);Cultivation project of National Natural Science Foundation of Guizhou Medical University(No.19NSP003);the Education Department of Guizhou Province(No.QJH KY[2021]189);the Natural Science Foundation of Guizhou Province(No.QKHZYD[2022]4015);High-level Innovative Talents in Guizhou Province(Thousand Levels of Talent for Chunmao Yuan in 2018);“Light of the West”Talent Cultivation Program of Chinese Academy of Sciences for Chunmao Yuan(No.RZ[2020]82).
This study employed the α-glucosidase inhibitory activity model as an anti-diabetic assay and implemented a bioactivity-guided isolation strategy to identify novel natural compounds with potential therapeutic propert...
关键词:Hypericum sampsonii Chemical constituents Structure elucidation Α-GLUCOSIDASE Molecular docking 
Cellular Growth Dynamics Affects Allelopathic Activity in Coffee Cell Culture
《American Journal of Plant Sciences》2023年第8期857-868,共6页Muchamad Imam Asrori Shinjiro Ogita 
Cellular growth dynamics and allelopathic activity in coffee cell cultures were examined as follows: First, we compared allelopathic activity of seven woody plant calli, Coffea canephora, Derris indica, Ficus carica L...
关键词:Allelopathic Activity Coffea canephora CALLUS Growth Phase Sandwich Method Bioassay 
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