TRANSGENE

作品数：115被引量：276H指数：8
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相关领域：农业科学生物学更多>>
相关作者：朱祯李聪吕川根张启军李仕贵更多>>
相关机构：江苏省农业科学院中国科学院遗传与发育生物学研究所四川省农业科学院四川农业大学更多>>
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Improvement in Tol2 transposon for efficient large-cargo capacity transgene applications in cultured cells and zebrafish(Danio rerio)被引量：1
《Zoological Research》2024年第3期567-574,共8页Peng-Cheng Wang Hao Deng Rang Xu Jiu-Lin Du Rongkun Tao 
supported by the National Science and Technology Innovation 2030 Major Projects(2021ZD0202200);National Natural Science Foundation of China(32171090,81970264);Shanghai Science and Technology Commission(21ZR1482600);2023 Youth Innovation Promotion Association CAS。
Most viruses and transposons serve as effective carriers for the introduction of foreign DNA up to 11 kb into vertebrate genomes.However,their activity markedly diminishes with payloads exceeding 11 kb.Expanding the p...
关键词：ZEBRAFISH Tol2 transposase TRANSGENE Large payload Synthetic biology 
Optimized protoplast isolation and transfection with a breakpoint:accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot
《aBIOTECH》2024年第2期151-168,共18页Debasmita Panda Subhasis Karmakar Manaswini Dash Swagat Kumar Tripathy Priya Das Sagar Banerjee Yiping Qi Sanghamitra Samantaray Pradipta Kumar Mohapatra Mirza J.Baig Kutubuddin A.Molla 
support from the University Grant Commission(UGC);funding support from the Science and Engineering Research Board(SERB)-National Post-Doctoral Fellowship.;funding support from the Department of Biotechnology(DBT),Government of India-JRF program.MD,SB,MJB;funding from the Indian Council of Agricultural Research(ICAR),New Delhi,in the form of the Plan Scheme-‘Incentivizing Research in Agriculture’project;support from the Director,NRRI.
The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target genomic loci.Software-predicted guide RNAs(gRNAs)often fail to induce ...
关键词：PROTOPLAST Genome editing Transgene-free edited plants Rice CHICKPEA 
Barley chitinase genes expression revamp resistance against whitefly (Bemisia Tabaci) in transgenic cotton (Gossypium hirsutum L.)
《Journal of Cotton Research》2024年第1期90-100,共11页BASHIR Samina YAQOOB Amina BASHIR Rohina BUKHARI Shanila SHAHID Naila AZAM Saira BAKHSH Allah HUSNAIN Tayyab SHAHID Ahmad Ali RAO Abdul Qayyum 
Background Chitinase is an enzyme that hydrolyzes chitin,a major component of the exoskeleton of insects,including plant pests like whiteflies.The present study aimed to investigate the expression of chemically synthe...
关键词：CHITINASE Cotton White fly TRANSGENE BIOASSAY 
Characterization of genetic humanized mice with transgenic HLA DP401 or DRA but deficient in endogenous murine MHC classⅡgenes upon Staphylococcus aureus pneumonia被引量：1
《Animal Models and Experimental Medicine》2023年第6期585-597,共13页Feng Li Bowen Niu Lingling Liu Mengmin Zhu Hua Yang Boyin Qin Xiuhua Peng Lixiang Chen Chunhua Xu Xiaohui Zhou 
National Science and Technology Major Project,Grant/Award Number:2016YFD0500208,2017ZX10304402-001-012 and 2017ZX10304402-001-006;Shanghai Science and Technology Commission“R&D public service platform and institutional capacity improvement project”,Grant/Award Number:21DZ2291300;Shanghai Public Health Clinical Center projects,Grant/Award Number:KY-GW-2021-39,KY-GW-2019-19 and KY-GW-2019-11。
Background:Staphylococcus aureus can cause serious infections by secreting many superantigen exotoxins in“carrier”or“pathogenic”states.HLA DQ and HLA DR humanized mice have been used as a small animal model to stu...
关键词：HLA DP401 HLA-DRA humanized mice MHC II Staphylococcus aureus pneumonia TRANSGENE 
Production of marker-free transgenic plants from mature tissues of navel orange using a Cre/loxP site-recombination system
《Horticultural Plant Journal》2023年第3期473-480,共8页Yongrui He Lanzhen Xu Aihong Peng Tiangang Lei Qiang Li Lixiao Yao Guojin Jiang Shanchun Chen Zhengguo Li Xiuping Zou 
supported by the Fundamental Research Funds for the Central Universities (Grant No. XDJK 2018B016);the National Natural Sciences Foundation of China (Grant No. 31972393);he earmarked fund for China Agriculture Research System (Grant No. CARS-26);the Natural Science Foundation of Chongqing (Grant No. cstc2020jcyj-msxmX1064)。
Genetic transformation with mature material as the explants could shorten the transgenic period and avoid seed dependence compared with genetic transformation using the epicotyl seedling stem segments as the receptor....
关键词：Citrus sinensis Osbeck Mature stem segment MARKER-FREE TRANSGENE Bud micrografting 
A novel hybrid seed production technology based on a unilateral cross-incompatibility gene in maize被引量：4
《Science China(Life Sciences)》2023年第3期595-601,共7页Darun Cai Zhaogui Zhang Li Zhao Juan Liu Huabang Chen 
supported by the National Natural Science Foundation of China(32101725,32172058)。
Hybrid seed production technology(SPT)using genic recessive male sterility is of great importance in maize breeding.Here,we report a novel SPT based on a maize unilateral cross-incompatibility gene Zm Ga1F with an ext...
关键词：ZmGa1F pollen tube growth genic male sterility seed production technology transgene transmission rate 
See the color,see the seed:GmW1 as a visual reporter for transgene and genome editing in soybean
《The Crop Journal》2023年第1期311-315,共5页Li Chen Shan Yuan Yupeng Cai Weiwei Yao Qiang Su Yingying Chen Jialing Zhang Wensheng Hou 
supported by the Agricultural Science and Technology Innovation Program of Chinese Academy of Agriculture Sciences(S2022ZD03)。
A fast and efficient recognition method of transgenic lines will greatly improve detection efficiency and reduce cost.In this study,we successfully identified the transgenic soybean plants by the color.We isolated a G...
关键词：SOYBEAN GmW1 COLOR Transgenic lines Genome editing 
Making Use of Plant uORFs to Control Transgene Translation in Response to Pathogen Attack被引量：3
《BioDesign Research》2022年第1期381-394,共14页Gan Ai Jin Liu Xiaowei Fu Tianli Li Hai Zhu Ying Zhai Chuyan Xia Weiye Pan Jialu Li Maofeng Jing Danyu Shen Ai Xia Daolong Dou 
the National Natural Science Foundation of China(31625023,31721004,and 32072507);the Fundamental Research Funds for the Central Universities(KYT202001).
Reducing crop loss to diseases is urgently needed to meet increasing food production challenges caused by the expanding world population and the negative impact of climate change on crop productivity.Disease-resistant...
关键词：EXPRESSING CROPS IMPAIRED 
Paternal transgenerational nutritional epigenetic effect:A new insight into nutritional manipulation to reduce the use of antibiotics in animal feeding
《Animal Nutrition》2022年第4期142-151,共10页Xinyi Li Mengya Wang Shimin Liu Xiaodong Chen Yu Qiao Xiaojun Yang Junhu Yao Shengru Wu 
National Natural Science Foundation of China(31902184);Shaanxi Provincial Science and Technology Association Young Talents Lifting Program Project(20220203)。
The use of antibiotics in animal feeding has been banned in many countries because of increasing concerns about the development of bacterial resistance to antibiotics and potential issues on food safety.Searching for ...
关键词：Growth NUTRITION Immune Transgenerational epigenetic Antibiotic replacement 
A transgene-free method for rapid and efficient generation of precisely edited pigs without monoclonal selection被引量：10
《Science China(Life Sciences)》2022年第8期1535-1546,共12页Kui Xu Xiuling Zhang Zhiguo Liu Jinxue Ruan Changjiang Xu Jingjing Che Ziyao Fan Yulian Mu Kui Li 
supported by the National Natural Science Foundation of China (32072690);the Major Scientific Research Tasks for Scientific and Technological Innovation Projects of the Chinese Academy of Agricultural Sciences (CAAS-ZDRW202006);the Science, Technology and Innovation Commission of Shenzhen Municipality (JCKYZDKY202009);the National Transgenic Breeding Project (2016ZX08010-004);the National Transgenic Breeding Project (2016ZX08006-001);the Agricultural Science and Technology Innovation Program (ASTIP-IAS05)。
Gene-edited pigs for agricultural and biomedical applications are typically generated using somatic cell nuclear transfer(SCNT).However, SCNT requires the use of monoclonal cells as donors, and the time-consuming and ...
关键词：dual-sg RNA CRISPR-Cas9 ribonucleoproteins transgene-free without monoclonal selection cloned pig 
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