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作品数：19被引量：40H指数：4
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相关领域：农业科学生物学更多>>
相关作者：周红艳肖桂芳朱祯李旭刚魏晓丽更多>>
相关机构：北京师范大学中国科学院遗传与发育生物学研究所浙江师范大学湖北省农业科学院更多>>
相关期刊：《Molecular Horticulture》《Oil Crop Science》《Journal of Integrative Agriculture》《Rice science》更多>>
相关基金：国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划重庆市自然科学基金更多>>
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Agrobacterium rhizogenes-mediated marker-free transformation and gene editing system revealed that AeCBL3 mediates the formation of calcium oxalate crystal in kiwifruit被引量：1
《Molecular Horticulture》2024年第1期574-588,共15页Pengwei Li Yiling Zhang Jing Liang Xufan Hu Yan He Tonghao Miao Zhiyin Ouyang Zuchi Yang Abdul Karim Amin Chengcheng Ling Yize Liu Xiuhong Zhou Xiaoran Lv Runze Wang Yajing Liu Heqiang Huo Yongsheng Liu Wei Tang Songhu Wang 
supported by the National Natural Science Foundation of China(U23A20204),Anhui Provincial Key Laboratory of Horticultural Crop Quality Biology,Startup Funding from Anhui Agricultural University,and the“Wanjiang Scholar”Program(Anhui Province).
The transformation and gene editing of the woody species kiwifruit are difficult and time-consuming.The fast and marker-free genetic modification system for kiwifruit has not been developed yet.Here,we establish a rap...
关键词：Marker-free transformation Agrobacterium rhizogenes CRISPR/Cas9 KIWIFRUIT AeCBL3 
Production of marker-free transgenic plants from mature tissues of navel orange using a Cre/loxP site-recombination system
《Horticultural Plant Journal》2023年第3期473-480,共8页Yongrui He Lanzhen Xu Aihong Peng Tiangang Lei Qiang Li Lixiao Yao Guojin Jiang Shanchun Chen Zhengguo Li Xiuping Zou 
supported by the Fundamental Research Funds for the Central Universities (Grant No. XDJK 2018B016);the National Natural Sciences Foundation of China (Grant No. 31972393);he earmarked fund for China Agriculture Research System (Grant No. CARS-26);the Natural Science Foundation of Chongqing (Grant No. cstc2020jcyj-msxmX1064)。
Genetic transformation with mature material as the explants could shorten the transgenic period and avoid seed dependence compared with genetic transformation using the epicotyl seedling stem segments as the receptor....
关键词：Citrus sinensis Osbeck Mature stem segment MARKER-FREE TRANSGENE Bud micrografting 
Efficient Gene Targeting in Maize Using Inducible CRISPR-Cas9 and Marker-free Donor Template被引量：4
《Molecular Plant》2020年第8期1219-1227,共9页Pierluigi Barone Emily Wu Brian Lenderts Ajith Anand William Gordon-Kamm Sergei Svitashev Sandeep Kumar 
CRISPR-Cas9 is a powerful tool for generating targeted mutations and genomic deletions.However,precise gene insertion or sequence replacement remains a major hurdle before application of CRISPR-Cas9 technology is full...
关键词：DONOR TEMPLATE INSERTION 
Marker gene excision in transgenic Brassica napus via Agrobacterium-mediated Cre/lox transient expression system
《Oil Crop Science》2020年第2期96-103,共8页Pandi Wang Xiaojuan Xiong Ping Fu Gang Wu Fang Liu 
supported by the National Program of Transgenic Variety Development of China(2018ZX08010-05B and 2019ZD080018);the Central Public-interest Scientific Institution Basal Research Fund;Major Research Project of CAAS Science。
Oilseed rape(Brassica napus L.)is one of the most important oil crops in the world.However,study on marker-free transgene of B.napus for bio-safety purpose is limited in this allotetraploid crop.In order to advance ma...
关键词：Brassica napus MARKER-FREE CRE/LOX Cross Auto-excision Herbicide resistance 
Generation of selectable marker-free soft transgenic rice with transparent kernels by downregulation of SSSⅡ-2被引量：4
《The Crop Journal》2020年第1期53-61,共9页Zhihao Xu Meimei Yu Youwei Yin Cenwen Zhu Wen Ji Changquan Zhang Qianfeng Li Honggen Zhang Shuzu Tang Hengxiu Yu Qiaoquan Liu 
supported by the Key Transgenic Breeding Program of China(2016ZX08001006,2016ZX08001002-003);the National Key Research and Development Program(2016YFD0102000);Yangzhou City Science and Technology Plan(YZ2017059);the National Natural Science Foundation of China(31872859);Jiangsu Agricultural Science and Technology Innovation Fund(CX181001);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
The amylose content(AC) of rice endosperm starch varies from 0 to 35%,and is associated with rice cooking and eating quality.Soft rice has low AC,generally between 6% and 15%,and its eating quality is high whether it ...
关键词：Rice(Oryza sativa L.) SSSⅡ-2 RNAi Starch quality SMF transgenic lines 
Genetic and agronomic traits stability of marker-free transgenic wheat plants generated from Agrobacterium-mediated co-transformation in T2 and T3 generations被引量：2
《Journal of Integrative Agriculture》2020年第1期23-32,共10页LIU Hui-yun WANG Ke WANG Jing DU Li-pu PEI Xin-wu YE Xing-guo 
the Ministry of Agriculture of China for the National Transgenic Research Program (2016ZX08010004);the Chinese Academy of Agricultural Sciences for the Agricultural Science and Technology Innovation Program (ASTIP-2060302-2-19)
Genetically modified wheat has not been commercially utilized in agriculture largely due to regulatory hurdles associated with traditional transformation methods. Development of marker-free transgenic wheat plants wil...
关键词：wheat marker-free transgenic plants fluorescence in situ hybridization genetic stability 
Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System被引量：4
《Rice science》2019年第2期129-132,I0025-I0027,共5页SHEN Lan DONG Guojun ZHANG Yu HU Guocheng ZHANG Qiang HU Guanglian XU Bo REN Deyong HU Jiang ZHU Li GAO Zhenyu ZHANG Guangheng GUO Longbiao ZENG Dali QIAN Qian 
supported by the Central Public-Interest Scientific Institution Basal Research Fund of China National Rice Research Institute (Grant No. 2017RG001-4)
Rice is an important food crop in China, and the development of hybrid rice is a crucial way to increase grain yield. The creation of dual-purpose nuclear-sterile lines for two-line hybrid breeding has become vital fo...
关键词：RICE CREATION MARKER-FREE 
Generation of marker-free transgenic rice using CRISPR/Cas9 system controlled by ?oral speci?c promoters
《Journal of Genetics and Genomics》2019年第1期61-64,共4页Junjie Wang Chun Wang Kejian Wang 
supported by the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences
Recently, the CRISPR/Cas9 system has been used as a powerful tool for genome editing in many species (Jinek et al., 2012;Cong et al., 2013;Wright et al., 2016;Li et al., 2017;Deng et al., 2018). The CR1SPR/Cas9 system...
关键词：the CRISPR/Cas9 SYSTEM EXPAND NUMEROUS precise base 
Development and drought tolerance assay of marker-free transgenic rice with OsAPX2 using biolistic particle-mediated co-transformation
《The Crop Journal》2017年第4期271-281,共11页Dan Feng Yanwei Wang Jinxia Wu Tiegang Lu Zhiguo Zhang 
supported by the National Major Project for Developing New GM Crops (2016ZX08001-003)
Abiotic stresses such as drought, salinity, and low temperature cause–losses in rice production worldwide. The emergence of transgenic technology has enabled improvements in the drought resistance of rice plants and ...
关键词：Rice PARTICLE bombardment-mediated CO-TRANSFORMATION MARKER-FREE Drought-tolerance 
Suicide Vector Construction of Haemophilus parasuis hhd B Gene Marker-free Deleted
《Animal Husbandry and Feed Science》2015年第6期354-357,共4页Song Shuai Li Miao Li Yan Jiang Zhiyong Cai Rujian Yang Dongxia Li Chunling 
Supported by Science and Technology Project of Guangdong Province(2013B020307002)
To construct the suicide vector of hhd B gene marker-free mutant in Haemophilus parasuis( HPS),two pairs of specific primers were designed and synthesized according to the hhd B gene upstream and downstream sequences ...
关键词：Haemophilus parasuis(HPS) hhd B gene Suicide vector 
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