Generation of marker-free transgenic rice using CRISPR/Cas9 system controlled by ?oral speci?c promoters  

作　　者：Junjie Wang Chun Wang Kejian Wang 

机构地区：[1]State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences

出　　处：《Journal of Genetics and Genomics》2019年第1期61-64,共4页遗传学报（英文版）

基　　金：supported by the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences

摘　　要：Recently, the CRISPR/Cas9 system has been used as a powerful tool for genome editing in many species (Jinek et al., 2012;Cong et al., 2013;Wright et al., 2016;Li et al., 2017;Deng et al., 2018). The CR1SPR/Cas9 system can not only be used as a useful technology to disrupt endogenous genes but also expand numerous other applications, such as precise base editing (Komor et al., 2016;Zong et al., 2017), regulation of gene expression (Gilbert et al., 2013), and gene replacement or insertion (Wang et al., 2017).

关 键 词：the CRISPR/Cas9 SYSTEM EXPAND NUMEROUS precise base 

分 类 号：R[医药卫生]