Multiplex analysis platform for evaluation of endocrine disruption of emerging contaminants against human steroid hormone receptors using autobioluminescent yeast bioassay:Application to bisphenols  

作　　者：Yuan Huang Wei Zhang Ruiguo Wang Zhiming Xiao John P.Giesy Liming Wu Xiaoou Su 

机构地区：[1]Institute of Quality Standards and Testing Technology for Agro-Products,Chinese Academy of Agricultural Sciences,Beijing,100081,China [2]Institute of Apicultural Research,Chinese Academy of Agricultural Sciences,Beijing,100093,China [3]Beijing Jingwa Agricultural Science and Innovation Center,Beijing,101206,China [4]Department of Veterinary Biomedical Sciences and Toxicology Centre,Western College of Veterinary Medicine,University of Saskatchewan,Saskatoon,SK,S7N 5B4,Canada [5]Department of Integrative Biology and Center for Integrative Toxicology,Michigan State University,East Lansing,MI,48824,USA [6]Department of Environmental Sciences,Baylor University,Waco,76706,USA

出　　处：《Emerging Contaminants》2024年第4期132-144,共13页新兴污染物(英文)

基　　金：supported by the National Key Research and Development Program of China(NO.2023YFD1301505,No.2017YFC1600303);the Agricultural Science and Technology Innovation Program of CAAS(CAAS-ASTIP-IQSTAP-04);Prof.Giesy was supported by the Canada Research Chair program,the 2012"High Level Foreign Experts"(#GDT20143200016)program,funded by the State Administration of Foreign Experts Affairs,the P.R.China to Nanjing University and the Einstein Professor Program of the Chinese Academy of Sciences and a Distinguished Visiting Professorship in the Department of Environmental Sciences,Baylor University in Waco,TX,USA.

摘　　要：Emerging synthetic or derivative chemicals have drawn global concern due to their potential endocrine-disrupting effects,either as agonists or antagonists or through indirect effects on enzymes of signal transduction pathways.Currently,in vitro screening models are limited to poor tolerance,robustness,high cost and need for sphisticated equipment or complex procedures that require high levels of expertise.Herein,a multiplex analysis platform based on autobioluminescent yeast strains,including BLYhERaS,BLYhERbS,BLYhPRS,BLYhARS,BLYhMRS,and BLYhGRS,was developed for use in easy,rapid,robust,and sensitive screening for potential modulation of endocrine systems.Methods were applied to assess endocrine-disrupting effects of Bisphenol A(BPA)and its alternatives.Disrupting activities of bisphenols(BPs)varied greatly,and some BPA substitutes exerted more potent activities than those of BPA.Most BPs were primarily agonists of ERa and ERb,while others were antagonists to AR,PR,GR,and MR.BPP and BPM exhibited non-monotonic dose-response relationships toward ERa and ERb,and BHPF and BPG displayed converse disrupting activities to ERa and ERb.Overall,the bioassay system provides an efficient tool for comprehensive evaluation of endocrine-disrupting activities of emerging contaminants.

关 键 词：teroid hormone receptors YEAST Agonistic activity Antagonistic activity BISPHENOLS Endocrine-disrupting 

分 类 号：O62[理学—有机化学]