茶氨酸和厚朴提取物对7日龄小鸡分离应激过程的影响  

Effects of theanine and houpu extract in 7-day chick social separation-stress procedure

作　　者：杨秋生[1] 徐平湘[1] 李宇航[1] 姜山[2] 张旭[2] 薛明[1] 

机构地区：[1]首都医科大学化学生物学与药学院,北京100069 [2]首都医科大学生物工程学院,北京100069

出　　处：《中国中药杂志》2007年第19期2040-2043,共4页China Journal of Chinese Materia Medica

摘　　要：目的:研究茶氨酸对7日龄小鸡应激反应的影响,与厚朴提取物(25 mg.kg-1)合用是否具有协同作用。方法:茶氨酸(12.5,25,50 mg.kg-1腹腔注射30 min后,记录分离小鸡和未分离小鸡自主活动、分离发声和福尔马林诱导的疼痛反应的影响。结果:茶氨酸3个剂量和厚朴提取物(25 mg.kg-1)能显著减轻分离小鸡的悲鸣(P<0.05,P<0.01);茶氨酸25,50 mg.kg-1对分离应激引起的痛觉钝抑(analgesia)有对抗作用。各组对自主活动均无明显影响。结论:茶氨酸与厚朴单独应用可以缓解小鸡分离应激反应,提示对紧张、焦虑和抑郁症有潜在的治疗作用。不同剂量茶氨酸与厚朴提取物25 mg.kg-1合用协同作用不明显。Objective： To examine the abilities of theanine and houpu extracts （HE） to reverse behavioral indexes （separation vocalization, stress-induced analgesia and activity）. Method： 7-day-old chicks received IP injection of theanine and HE 30min before being tested in the presence of three social companions or in isolation for 3-min observation period. Dependent measures were： a） Chicks were placed into an infrared ray device to calculate their spontaneous activities by a computer program b） record the separation vocalizations for every chick, c） In the experiment of stress-induced analgesia, 50 uL of formalin （0. 1% ） was injected into the plantar of the animal foot to index stress-induced analgesia （i. e. foot-lift frequency, foot-lift duration and peck frequency）. Result： In the experiments, isolated chicks exhibited more vocalizations （P 〈0. 01 ） and fewer pain-related behaviors than non-isolated chicks （P 〈 0. 01 ）. Theanine （ 12. 5, 25, 50 mg·kg^-1 ） and HE （25 mg·kg^-1 ） decrease separately the tendency （dB）of the principal frequency （P 〈 0. 05, P 〈 0. 01 ） ; The stress induced analgesia can be reversed by theanine in 25, 50 mg · kg^-1. Both of the materials do not affect the spontaneous activities in this chick model without causing sedation. Conclusion： These results suggest that theanine and HE in the dosages may be useful in modulating anxiety states. They are seems no synergism in the chick model.

关 键 词：应激 茶氨酸 厚朴提取物 7日龄小鸡 分离试验 

分 类 号：R285.5[医药卫生—中药学]