二甲肼诱发大鼠大肠癌癌前病变模型的肠道双歧杆菌数量研究  

Quantification of intestinal bifidobacteria in the rats with precancerous lesions of colorectal cancer induced by DMH

作　　者：魏华[1] 张昕[2] 张晨虹[1] 王婷婷[1] 董立[3] 吴大正[4] 张梦晖[1] 庞小燕[1] 赵立平[1] 

机构地区：[1]上海交通大学生命科学技术学院微生物分子生态与生态基因组学实验室,上海200240 [2]山西大学生物技术研究所化学生物学与分子工程教育部重点实验室,山西太原030006 [3]山东省泰安市中心医院药剂科,山东泰安271000 [4]上海中医药大学中药研究所上海市高校中医内科学E-研究院,上海201203

出　　处：《中华肿瘤防治杂志》2007年第20期1524-1526,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：上海市科委科研基金资助项目(05DJ14009);上海市教委科研基金资助项目(E03008)

摘　　要：目的:探讨二甲肼(DMH)诱发的大肠癌癌前病变模型大鼠的肠道内双歧杆菌数量与大肠癌癌前病变的关系。方法:应用双歧杆菌属特异性引物实时定量PCR,对实验第3与第9周8只模型组大鼠以及同年龄的7只正常对照组大鼠肠道中的双歧杆菌数量进行检测。结果:第9周的正常对照组大鼠未见明显异常隐窝灶(aberrant crypts foci,ACF),同年龄的模型组平均每只大鼠大肠可形成(37.7±2.6)个ACF。第3周正常对照组与同年龄的模型组大鼠肠道双歧杆菌数量差异无统计学意义,P=0.652;第9周模型组大鼠肠道双歧杆菌数量低于同年龄的正常对照组,但差异无统计学意义,P=0.090。结论:肠道内双歧杆菌的数量与大鼠大肠癌癌前病变没有明显相关性。OBJECTIVE：To study the relationship between the quantification of intestinal bifidobacteria and precancerous lesions of colorectal cancer by using a rat model with precancerous lesions of colorectal cancer induced by 1, 2-dimethylhydrazine （DMH）. METHODS：The quantification of bifidobacteria of 7 control rats and 8 model rats was detected by using Bifidobacteriunz genus-specific real-time quantitative PCR in the third and ninth week, respectively. RESULTS：The rats of the control group had no aberrant crypts loci （ACF）, while every rat of the model group had （37.7± 2.6） ACF/colon in the ninth week in this experiment. No difference of the quantification of bifidobacteria was found between the control and model rats in the third week, P= 0. 652. In the ninth week, the quantification of the bifidobacteria of model rats was lower than that of the control group, but it showed no statistical difference, P 0. 090. CONCLUSION： There is no obvious relationship between the quantification of intestinal bifidobacteria and rats＇ precancerous lesions of colorectal cancer induced by DMH.

关 键 词：二甲基肼类/副作用 结直肠肿瘤/遗传学 疾病模型 动物 抗肿瘤药/药理学 

分 类 号：R735.34[医药卫生—肿瘤]