TRAF4蛋白在乳腺癌组织中的表达及意义  

Expression and Significance of TRAF4 Protein in Breast Carcinoma

作　　者：代文博[1] 郑英伟[1] 米小轶[1] 刘楠[1] 林红[2] 闫俊[2] 

机构地区：[1]中国医科大学病理学教研室,辽宁沈阳110001 [2]沈阳市第一人民医院病理科,辽宁沈阳110041

出　　处：《癌症》2007年第10期1095-1098,共4页Chinese Journal of Cancer

摘　　要：背景与目的:对肿瘤坏死因子受体相关因子4(tumor necrosis factor receptor-associated factor4,TRAF4)在乳腺癌中表达的研究存在争议。本研究探讨TRAF4在正常乳腺组织、乳腺癌组织及不同侵袭能力乳腺癌细胞系中的表达情况。方法:应用免疫组化方法检测TRAF4在70例乳腺癌组织和14例正常乳腺组织中的表达。应用Westernblot方法检测TRAF4在乳腺癌细胞系MDA-MA-231(高侵袭)和MCF-7(低侵袭)中的表达。结果:TRAF4在正常乳腺组织中呈浆、核阳性表达。其浆阳性率在正常乳腺组织(78.57%)、非浸润性导管癌(88.57%)和浸润性导管癌(91.43%)中逐渐增高,但差异无统计学意义(P>0.05)。而核阳性率在正常乳腺组织(64.28%)中显著高于非浸润性导管癌(28.57%,P<0.01),在非浸润性导管癌中高于浸润性导管癌(5.7%,P<0.05)。TRAF4总蛋白在乳腺癌高侵袭细胞系中的表达量高于低侵袭细胞系,但差异无统计学意义(P>0.05)。结论:TRAF4在乳腺癌细胞核中表达明显降低,与乳腺癌侵袭性相关。　BACKGROUND ＆ OBJECTIVE： The researches about the expression of tumor necrosis factor receptor-associated factor 4 （TRAF4） in breast cancer are disputable. This study was to investigate the expression of TRAF4 in normal breast, breast carcinoma tissue, and cell lines with different invasive abilities. METHODS： The expression of TRAF4 in 70 specimens of breast carcinoma and 14 specimens of normal breast tissues was detected by SP immunohistochemistry. The expression of TRAF4 in breast cancer cell lines, MDA-MB-231 with high invasive ability and MCF-7 with low invasive ability, was detected by Western blot. RESULTS： TRAF4 was expressed both in cell cytoplasm and nuclei in normal breast tissues. The cytoplasmic positive rates of TRAF4 were 78.57% in normal breast tissues, 88.57% in non-invasive ductal carcinoma, and 91.43% in invasive ductal carcinoma （P〉0.05）. The nuclear positive rate of TRAF4 was significantly higher in normal breast tissues than in non-invasive ductal carcinoma （64.28% vs. 28.57%, P〈0.01）, and higher in non-invasive ductal carcinoma than in invasive ductal carcinoma （28.57% vs. 5.70%,P〈0.05）. The protein level of TRAF4 was slightly higher in MDA-MB-231 cells than in MCF-7 cells （P〉0.05）. CONCLUSION： The nuclear expression of TRAF4 in breast carcinoma is suppressed, and correlated to the invasive ability of breast cancer.

关 键 词：乳腺肿瘤 肿瘤坏死因子受体相关因子-4 免疫组化 肿瘤侵袭 

分 类 号：R737.9[医药卫生—肿瘤]