多巴胺在聚变色酸2B膜电极上的电化学行为及其测定  

The electrochemical behavior and determination of dopamine at poly-chromotrope 2B film modified glassy carbon electrode

作　　者：庄茜[1] 陈敬华[1] 张少波[1] 罗红斌[1] 林新华[1] 

机构地区：[1]福建医科大学药学院药物分析系,福州350004

出　　处：《药物分析杂志》2007年第9期1403-1405,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家863计划(2006AA02Z4Z1);国家自然科学基金(20675015);福建省科技厅重点课题(200610016);福建省教育厅基金(JA04194;2005K051;2005K049)

摘　　要：目的:研究多巴胺(DA)在聚变色酸2B 膜(PCR2B)修饰电极上的伏安行为,建立测定 DA 含量的差示脉冲伏安法。方法:采用循环伏安法研究 DA 在膜修饰电极上的电化学行为,以差示脉冲伏安(DPV)对其含量进行测定。结果:聚变色酸2B 膜修饰电极对 DA 有明显的电催化作用。在 pH 5.0磷酸盐缓冲液(PBS)中,氧化峰电流与 DA 浓度在2.0×10^(-6)～1.0×10^(-5)mol·L^(-1)范围内呈良好的线性关系,检测限为3.2×10^(-7)mol·L^(-1)。结论:该修饰电极可有效消除针剂中其他组分对 DA测定的干扰,可用于实际样品中 DA 含量的直接测定。Objective ：To study the voltammetric behavior of dopamine （DA） at poly - chromotrope 2B （PCR2B） film modified electrode and establish differential pulse voltammetry（DPV） method for the determination of the concentration of DA. Method：The electrochemical behavior of DA at PCR2B film modified electrode was studied by cyclic voltammetry while the determination of DA was measured in DPV. Results：The modified electrode showed excellent electrocatalysis characteristics for DA. In pH 5.0 phosphate buffer solution （ PBS）, the relationship between oxidation peak current and the concentration of DA was linear in the range of 2. 0 × 10^ -6 - 1.0 × 10 ^-5 mol · L^-1. The detection limit has been estimated as 3.2 × 10^-7 mol · L^-1. Conclusion：The interference of other components in the preparation can be efficiently eliminated on the modified electrode, and this method can be used for the determination of DA in sample.

关 键 词：变色酸2B 修饰电极 电化学行为 多巴胺 

分 类 号：R917[医药卫生—药物分析学]