犬瘟热病毒蓝狐分离株融合蛋白基因的序列分析  

Nucleotide sequence analysis of fusion protein gene of canine distemper virus isolated from alopex

作　　者：苏凤艳[1] 丁庆东[1] 宗春苗[1] 王卓聪[1] 王春雨[1] 王全凯[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118

出　　处：《中国预防兽医学报》2007年第10期764-767,782,共5页Chinese Journal of Preventive Veterinary Medicine

摘　　要：根据已发表的犬瘟热病毒(Canine distemper virus,CDV)Onderstepoort株序列设计1对引物,RT-PCR法扩增出约1 000 bp的融合蛋白基因片段,通过T-A克隆技术,将PCR产物克隆至pMD18-T载体。序列分析表明CDV蓝狐分离株F蛋白基因片段由1 044 bp组成,编码348个氨基酸,与其它CDV25259株、2544-Han95株、A75-17株、DOGDK91C株、5804P株、ONP株、PDV-2株核苷酸序列同源性分别为94.5%、94.1%、94.7%、94.2%、94.2%、97.6%和94.9%;氨基酸序列的同源性分别为98.6%、97.1%、98.0%、98.0%、98.3%、97.7%和98.6%;与其它CDV毒株相比,蓝狐分离株存在核苷酸缺失突变(1 030位～1 038位)和氨基酸缺失突变(344位～348位)。The fusion protein （F） gene of canine distemper virus （CDV） was amplified by RT-PCR from the genomic RNA isolated from alopex. The PCR product was clones into pMD18-T vector and sequenced. The fusion gene of CDV-A1 strain shared a nucleotide identity of 94.5 %, 94.1%, 94.7 %, 94.2 %, 94.2 %, 97.6 %, 94.9 % and an amino acid identity of 98.6 %, 97.1%, 98.0 %, 98.0 %, 98.3 %, 97.7 %, 98.6 % with those of strain 25259, 2544Han95, A75-17, DOGDK91C, 5804P, ONP, PDV-2, respectively. There was a deletion at nucleotide position of 1 030-1 038 and amino acid position of 344-348 in the F gene of the alopex isolate.

关 键 词：蓝狐 犬瘟热病毒 融合蛋白基因 序列分析 

分 类 号：Q781[生物学—分子生物学] S852.65[农业科学—基础兽医学]