树突状细胞激活效应细胞的最佳效靶比及其体外杀伤活性的研究  

STUDY ON THE OPTIMAL EFFICIENT RATIO ON TARGET CELLS AND THE ANTITUMOR ACTIVITY OF EFFECT CELLS STIMULATED BY DENDRITIC CELLS IN VITRO

作　　者：张春燕[1] 刘剑勇[1] 张志明[1] 张力图[1] 唐凯[1] 利基林 叶司原[1] 

机构地区：[1]广西医科大学附属肿瘤医院,南宁530021

出　　处：《广西医科大学学报》2007年第4期493-496,共4页Journal of Guangxi Medical University

基　　金：广西科学研究与技术开发计划攻关项目(No.桂科攻0143052)

摘　　要：目的:探讨树突状细胞(DC)激活肿瘤浸润淋巴细胞(TIL)和小鼠脾淋巴细胞(小鼠脾LC)的最佳效靶比并观察被有效激活的TIL和小鼠脾LC等对小鼠H22肝癌细胞的体外杀伤作用。方法:从荷瘤小鼠四肢长骨提取DC,联合应用GM-CSF、IL-4和小鼠H22肝癌细胞全细胞性抗原致敏DC,然后依据不同的激活效靶比用致敏DC体外激活TIL、小鼠脾LC,分别检测、比较被不同程度激活的TIL、小鼠脾LC以及未激活的小鼠脾LC、未激活的TIL对小鼠H22肝癌细胞的杀伤活性。结果:①当DC∶靶细胞(E/T)为1∶400和1∶200时,所激活的TIL或小鼠脾LC的杀伤活性较弱;当E/T为1∶100时,杀伤活性有较明显的提高,与前两者比较,差异有统计学意义(P<0.05);当E/T=1∶50时,杀伤活性增加,与前三者比较差异均有统计学意义(P<0.01或P<0.05);而当E/T=1∶25、1∶12.5和1∶6.25时,杀伤活性与E/T=1∶50时比较无明显变化(P>0.05);②当E/T=1∶50时,未经DC激活的小鼠脾LC(A组)杀伤率为(9.73±1.40)%,未经DC激活的TIL(B组)和DC激活的小鼠脾LC(C组)杀伤率分别为(50.91±2.36)%和(49.70±2.70)%,DC激活的TIL(D组)的杀伤率为(73.49±2.46)%。A组与其他组分别比较,差异均有统计学意义(P<0.01);B组和C组比较差异无统计学意义(P>0.05);D组与其他各组分别比较,差异均有统计学意义(P<0.01)。结论:①当E/T=1∶50时,DC能很好的发挥抗原提呈作用,使小鼠脾LC和TIL得以充分激活;②充分激活的小鼠脾LC或TIL对小鼠H22肝癌细胞的体外杀伤活性较未激活的小鼠脾LC或未激活的TIL均具有明显的提高,尤以激活的TIL的杀伤活性最强。Abstract Objective： To investigate the optimal efficient ratio of efficacy to target （E/T） and the antitumor activity of tumor-infiltrating lymphocytes（TILs） and the mouse＇s splenocyte lymphocytes stimulated by dendritic cells（DCs） on H22 cells in vitro. Methods： DCs were isolated from mouse bone marrow and stimulated by granuloeyte/ macrophage colony stimulating factor （GM-CSF）, interleukin-4 （IL-4） and tumor cellular antigen. Then TILs and the mouse＇s splenocyte lymphocytes were stimulated by the activation DCs under different E/T conditions, and their anti-tumor activity on H22 cells in vitro were observed respectively. Results：（1）At E/T of 1 ： 400 and 1 ： 200 respectively, the anti-tumor activity of TILs or the mouse＇s splenocyte lymphocytes stimulated by DCs were low respectively; at E/T of 1 ： 100, the antitumor activity increased, and the difference was significant as compared with those at E/T of 1 ： 400 and 1 ： 200 respectively （ P 〈0.05）; at E/T of 1 ： 50, the anti-tumor activity increased remarkably,and there was significant difference as compared with those at E/T of 1 ： 400 , 1 ： 200 and 1 ： 100 respectively （ P〈 0. 01 or P 〈0.05） ; but at E/T of 1 ： 25, 1 ： 12.5 and 1 ： 6.25 respectively, the anti-tumor activity almost had no change as compared with that at E/T of 1 ： 50（ P 〉0.05）. （2）at E/T of 1 ： 50, the anti-tumor activity of the mouse＇s splenocyte lymphocytes not stimulated by DCs（group A） was（9. 73±1.40）%, while the antitumor activity of the TILs not stimulated by DCs （group B） and the mouse＇s splenocyte lymphocytes stimulated by DCs （group C） were （50.91 ± 2.36） % and （49.70±2.70） % respectively. The antitumor activity of the TILs stimulated by DCs （group D） was（73.49± 2.46）%. the anti-tumor activity o{ group A as compared with those of other three groups respectively had a significant difference （ P 〈0.01）,there wasn＇t a significant difference between the an

关 键 词：树突状细胞 肿瘤浸润淋巴细胞 效靶比 杀伤活性 

分 类 号：R730.5[医药卫生—肿瘤] R-332[医药卫生—临床医学]