河南和新疆地区HIV-1毒株gp120和gag基因的变异分析  

An analysis of gp120 and gag gene variation of HIV-1 strains isolated from Henan and Xinjiang in China

作　　者：宋艳辉[1] 邢辉[2] 王哲[3] 张远志[4] 孙峰[4] 何翔[2] 陈建平[2] 邵一鸣 

机构地区：[1]中国科学院武汉病毒研究所 [2]中国疾病预防控制中心性病艾滋病预防控制中心传染性疾病预防与控制国家重点实验室,北京100050 [3]河南省疾病预防控制中心,郑州450003 [4]新疆维吾尔自治区疾病预防控制中心,乌鲁木齐830001

出　　处：《中国艾滋病性病》2007年第5期405-408,共4页Chinese Journal of Aids & STD

基　　金：与HIV疫苗研制相关的HLA分型和表位作图研究(N01-A1-30024);"十五"国家科技攻关项目(2001BA705B02和2004BA719A01)

摘　　要：目的分析中国河南和新疆地区艾滋病病毒Ⅰ型(HIV-1)毒株gp120和gag基因全长序列的变异特征。方法PCR扩增河南和新疆HIV-1确认阳性样本前病毒DNA gp120和gag全长基因,测序得到40份gp120和41份gag基因序列,用GCG软件包等软件进行分析。结果河南省HIV-1毒株为B′亚型,新疆维吾尔自治区HIV-1毒株为CRF07 BC亚型。B′亚型毒株gp120基因中变异程度最大的区段是V5区段,最小的是C1区段;CRF07BC亚型毒株中变异最大的是V1区段,最小的是C4区段。两种亚型毒株gp120和gag基因的Ks/Ka值<1(P<0.05)。结论对于病毒基因全长序列的分析可以更多地反映病毒的特性。gp120基因各个区段在B′和CRF07BC亚型毒株中的变异程度不一致。B′和CRF07 BC亚型毒株的gp120基因和gag基因都受到正向选择压力的作用。Objective To analyze gpl20 and gag genes variation of HIV-1 strains isolated from Henan and Xinjiang in China. Method Full length sequences of gpl20 and gag of provirus DNA of the isolated strains were amplified by nested- PCR.Forty gpl20 and 41 gag gene sequences thus obtained were analyzed by BLAST, MEGA and GCG soft wares. Results B＇subtype strains were predominant in Henan province while CRF07-BC subtype strains were circulating in Xinjiang Uygur Autonomous Region. The gpl20 gene V5 region was more variable and C1 was more stable in B＇ subtype strains whereas the V1 was more variable and CA was more stable in CRF07-BC subtype strains. The Ks/Ka ratios of gpl20 and gag genes were lower than 1 （P 〈 0.05）. Conclusions Significant biological features of HIV-1 can be displayed by analyzing full length sequences of virus genes. The gene variations of gpl20 gene of B＇ and CRF07-BC subtype strains were not consistent. The changes of gpl20 and gag genes were under positive selective pressure.

关 键 词：艾滋病病毒Ⅰ型 基因距离 选择压力 系统进化树 

分 类 号：R373.9[医药卫生—病原生物学]