温郁金提取液对化学致癌剂致大鼠胃癌的预防作用  

Chemoprevention of gastric cancer in rats by a wetdistillation extract of radix curcumae

作　　者：徐磊[1] 吕宾[1] 俞林峰[1] 

机构地区：[1]浙江中医药大学附属第一医院消化科,浙江省杭州市310006

出　　处：《世界华人消化杂志》2007年第24期2589-2592,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨温郁金蒸馏提取液对化学致癌剂N-甲基-N-′-硝基-亚硝基胍(MNNG)诱导Wistar大鼠胃癌的作用.方法:♂Wistar大鼠80只随机分为A,B,C,D,E5组,每组16只,A组为对照组,B组为模型组,分别自由饮用净化水和100 mg/L MNNG液同时予生理盐水5 mL ig 1次/d;C组为塞来昔布组,饮用MNNG同B组,同时予10 mg/kg塞来昔布5 mL ig 1次/d;D为低剂量郁金组,E组为高剂量郁金组,饮用MNNG同B组,同时分别予1和2 kg/L温郁金水蒸气蒸馏提取液5 mL ig 1次/d,持续40 wk.41 wk后处死动物,分组拍照片并行组织病理切片比较大体和组织学变化.结果:实验期间大鼠死亡32只,余48只完成实验.A,B,C,D,E组胃癌发生率分别为0.0% (0/16),44.4%(7/16),12.5%(2/16),12.5%(2/16),12.5%(2/16);与B组相比,C,D,E组胃癌发生率(P<0.05)和肿瘤体积(4.4±0.8 cm^3,6.6±0.6 cm^3,3.4±0.6 cm^3 vs 11.5±1.7 cm^3;P<0.01)显著降低;C,D,E组相互比较差异无显著性(P= 1.000).结论:温郁金水蒸气蒸馏提取液对MNNG诱导的大鼠胃癌有预防作用.AIM： To study the chemopreventive effects of a wet-distillation extract of radix curcumae in a wistar rat model of stomach carcinogenesis induced by N-methyl-N＇-nitro-N-nitrosoguani- dine （MNNG）. METHODS： Eighty 6-week-old male wistar rats were randomly divided into 5 groups of 16 animals each. Rats were given water and 5 mL normal saline daily （group A）; MNNG and 5 mL normal saline daily （group B）; 10 mg/kg celecoxib daily （group C）; 1 kg/L wet-distillation extract of radix curcumae, 5 mL daily （group D）; and 2 kg/L wet-distillation extract of radix curcumae, 5 mL daily （group E）. Animals in groups B-E dranh water containing MNNG （100 rag/L） and were given 100 g/L NaC1 in the initial 6 wk. All animals received treatment for 40 weeks and were then sacrificed at week 41. Gastric tumors were evaluated histologically. RESULTS： Thirty-two rats died during the experiment and 48 completed the experiment.Incidence of gastric cancer was 0.0% in Group A, 44.4% in Group B, and 12.5% in Groups C-E. We found that there was a statistically significant difference in the incidence of tumor formation （P 〈 0.05）, and volume （4.4±0.8 cm^3, 6.6 ± 0.6 cm^3, 3.4 ± 0.6 cm^3 vs 11.5 ± 1.7 cm^3; all P 〈 0.01） in Groups C-E compared with Group B. CONCLUSION： The wet-distillation extract of radix curcumae can reduce the incidence of gastric cancer and tumor growth in rats.

关 键 词：温郁金 胃癌 化学预防 

分 类 号：R735.2[医药卫生—肿瘤]