重组溶葡球菌酶治疗小型猪皮肤烫伤MRSA感染  

Anti-infection effect of recombinant lysostaphin on mini porcine model of skin localized burn wound infection by MRSA

作　　者：杨信怡[1] 张伟新[1] 李聪然[1] 娄人慧[1] 刘京芳[1] 陈慧贞[1] 母立新[1] 黄青山[2] 蒋建东[1] 游雪甫[1] 

机构地区：[1]中国医学科学院中国协和医科大学医药生物技术研究所药理室,北京100050 [2]复旦大学生命科学学院遗传研究所遗传工程国家重点实验室,上海200433

出　　处：《中国新药杂志》2007年第18期1477-1481,共5页Chinese Journal of New Drugs

基　　金：国家十五重大科技专项资助项目(2003AA2Z347D);中国高科集团股份有限公司生物医药专项基金

摘　　要：目的:评价重组溶葡球菌酶对实验性皮肤烫伤耐甲氧西林金葡菌(MRSA)感染的抗感染作用。方法:用中国实验用小型猪建立局部皮肤烫伤后MRSA感染模型,不同处理组创面治疗3 d或7 d后,进行痂下组织活菌计数及组织病理学观察,评价药物抗菌疗效。结果:重组溶葡球菌酶对烫伤皮肤的感染细菌有较好的清除作用,治疗后d 4和d 8高浓度(1 000 U.mL-1)组创面活菌计数[(5.94±0.61)和(4.03±0.75)lg CFU.g-1组织]均明显低于空白对照组[(7.48±0.33)和(6.32±0.60)lg CFU.g-1组织]和阳性药SD-Ag组创面[(7.44±0.19)和(5.29±0.40)lg CFU.g-1组织],P<0.05或P<0.01;治疗后d 8,中浓度(330 U.mL-1)组创面活细菌计数[(5.10±0.29)lg CFU.g-1组织]明显低于空白对照组创面[(6.32±0.60)lg CFU.g-1组织],P<0.05。组织病理学观察也反映了重组溶葡球菌酶的良好疗效。结论:重组溶葡球菌酶对MRSA引起的实验性皮肤烫伤感染有良好的抗感染作用。Objective： To evaluate the anti-infection effect of recombinant lysostaphin （rLspn） on China experimental mini porcine model of skin localized burn wounds infected by MRSA. Method： An experimental porcine model of skin localized burn wound infection was established. Wounds infected by MRSA were grouped and treated by topical use of saline, SD-Ag, or three different doses of rLspn for 3 or 7 days. The effects of the treatments were evaluated by measuring the amount of bacteria in the wounds and the microscopic observations of the wound tissues on day 4 and day 8. Results： rLspn could effectively reduce the amount of bacteria in the wounds. The amount of bacteria in the wounds in high dose group （ 1 000 U·mL^-1 of rLspn [ （5.94 ± 0.61 ） and （4.03 ± 0.75 ） lg CFU·g^-1 tissue ] was significantly lower than that in control [ （7.48 ± 0.33 ） and （6.32 ± 0.60） lg CFU·g^-1 tissue ] or SD-Ag groups [ （7.44 ±0.19） and （5.29 ±0.40） lg CFU·g^-1 tissue] on day 4 and day 8 of treatment, P 〈 0.05 or P 〈0.01. The amount of bacteria in the wounds in medium dose group（330 U·mL^-1）of rLspn [ （5.10 ±0.29） lg CFU·g^-1 tissue] was significantly lower than that in control group [ （6.32 ±0.60） lg CFU·g^-1 tissue ] on day 8 of treatment, P 〈 0.05. These results were also supported by the microscopic observations of the wound tissues. Conclusion： rLspn could effectively control the infection caused by MRSA in burn wound of porcine.

关 键 词：重组溶葡球菌酶 烫伤感染模型 中国实验用小型猪 耐甲氧西林金葡菌 抗菌效应 

分 类 号：R978[医药卫生—药品] R965.2[医药卫生—药学]