高效液相色谱法测定脑络通胶囊中维生素B_6的含量  

Determination of vitamin B_6 in Naoluotong capusules by HPLC

作　　者：苏明武[1] 袁征[1] 尹久[1] 李志浩[1] 

机构地区：[1]湖北中医学院药物分析教研室,武汉430061

出　　处：《中国新药杂志》2007年第18期1505-1507,共3页Chinese Journal of New Drugs

摘　　要：目的：建立高效液相色谱法测定脑络通胶囊中维生素B6含量的方法,并计算含量均匀度。方法：采用高效液相色谱法,Dikma C18柱（250 mm×4.6 mm,5μm）;流动相：甲醇-0.06%庚烷磺酸钠水溶液（含1.2%冰醋酸与0.12%三乙胺）（13∶87）;流速：1.0 mL ·min^-1;检测波长：290 nm;柱温：30℃。结果：维生素B6在0.156-0.780μg范围内呈良好的线性关系,回收率为98.9%,RSD为1.8%。含量均匀度符合《中华人民共和国药典》规定。结论：本方法简便、快速、准确,可用于脑络通胶囊中维生素B6的含量测定。Objective：To develop an HPLC method for assaying vitamin B6 and the uniformity in Naoluotong capsules. Methods：A Dikma C18 column（250 mm × 4.6 mm,5μm） was used for chromatographic separation at 30℃. The mobile phase consisted of methanol-0.06% sodium heptanesulfonate containing 1.2% acetic acid glacial and 0.12% triethylamine（13：87） , The flow rate was 1.0 mL·min^-1 and detection wavelength was set at 290 nm. Results：There was good linearity in the range of 0. 156 0.780μg. The average recovery was 98.9% ,and RSD was 1.8%. Conclusion：The method is simple, accurate, and can be used for determination of vitamin B6 in Naoluotong capusules.

关 键 词：脑络通胶囊 维生素B6 含量均匀度 高效液相色谱法 

分 类 号：R286.2[医药卫生—中药学] R927.2[医药卫生—中医学]