损伤大鼠L_5脊神经及其前后根对痛行为的影响  

THE EFFECT OF LUMBAR 5 SPINAL NERVE AND ITS VENTRAL ROOT OR DORSAL ROOT INJURY ON THE PAIN RELATED BEHAVIORS IN RATS

作　　者：许继田[1] 涂会引[1] 樊红琨[1] 

机构地区：[1]郑州大学医学院生理学教研室,郑州450052

出　　处：《中国疼痛医学杂志》2007年第5期280-284,共5页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金资助课题编号:30570599

摘　　要：目的:观察大鼠腰5脊神经和脊神经根不同部位损伤对诱导神经病理性疼痛的不同作用。方法:采用腰5脊神经结扎加切断(lumbar5 spinal nerve ligation,L5 SNL)、腰5前根切除(lumbar5 ventral rhizotomy,L5 VR)和腰5背根切除(lumbar5 dorsal rhizotomy,L5 DR)诱导大鼠痛觉过敏,结合痛行为学测试观察病理性疼痛的发展过程。结果:(1)L5SNL可引起大鼠病理性疼痛。双侧后肢50%撤足阈值(paw withdrawal threshold,PWT)和撤足潜伏期(paw withdrawal latency,PWL)于术后1d明显下降,痛觉过敏的症状,在同侧后肢持续了5周,在对侧后肢也保持3周。(2)L5 VR也可诱导大鼠产生病理性疼痛。双侧后肢50%PWT和PWL于术后1d明显降低,并维持到了术后第5周。(3)L5DR没有引起大鼠产生痛觉过敏症状。与术前基础值和假手术组比较,L5DR后50%PWT和PWL均无明显变化。结论:选择性损伤运动纤维和损伤脊神经均能诱导大鼠产生病理性疼痛,但脊神经背根损伤不引起痛觉过敏。To investigate the effect of different site and type of spinal nerve injury on the development of neuropathic pain. Methods： The lumbar 5 spinal nerve ligation plus transection and lumbar 5 ventral or dorsal rhizotomy was used. To elucidate the development of neuropathic pain, behavior test was performed before and after the animal operation. Results ： （ 1 ） Lumbar 5 spinal nerve ligation plus transection induced neuropathic pain. The 50% paw withdrawal threshold （PWT） and paw withdrawal latency （PWL） were significantly decreased l d after operation. The pain-related behaviors in ipsilateral hind paw was lasted for 5 weeks and maintained for 3 weeks in contralateral hind paw . （2） Lumbar 5 ventral rhizotomy led to mechanical allodynia and thermal hyperalgesia in bilateral hind paws. The significant decrease of PWT and PWL was observed 1 d after operation and lasted for nearly 5 weeks. （3） Lumbar 5 dorsal rhizotomy failed to induce neuropathi operated baseline and sham operated group, the PWT and PWL in c pain behaviors. Compared with prebilateral hind paws were not changed following L5 DR. Conclusion： The above data suggest that spinal nerve injury and even selective injury to motor fiber induced neuropathic pain, whereas the dorsal rhizotomy didn＇t lead to pain related behaviors.

关 键 词：诱导大鼠 背根损伤 脊神经根 神经病理性疼痛 痛行为 痛觉过敏 后根 过敏症状 

分 类 号：R741[医药卫生—神经病学与精神病学]