小鼠骨癌痛模型的建立及痛行为学和骨损害的观察  

THE MOUSE MODEL OF BONE CANCER PAIN AND EVALUATION OF PAIN BEHAVIOR AND BONE DESTRUCTION

作　　者：马正良[1] 夏小萍[1] 朱巍[1] 业光衡[1] 韩鹂[2] 费强[3] 

机构地区：[1]南京大学医学院附属鼓楼医院麻醉科,南京210008 [2]南京大学医学院附属鼓楼医院科研部,南京210008 [3]南京大学医学院附属鼓楼医院放射科,南京210008

出　　处：《中国疼痛医学杂志》2007年第5期288-292,共5页Chinese Journal of Pain Medicine

基　　金：南京市科技局(200504019-4);江苏省卫生厅开放课题(卫WK200501)共同资助

摘　　要：目的:在小鼠骨癌痛模型的基础上观察小鼠行为学和骨质损害的程度。方法:将2×105个NCTC2472小鼠纤维肉瘤细胞注射到C3H/HeJ小鼠右侧股骨远端骨髓腔内制作骨癌痛动物模型。分别于注射后1、3、5、7、10、14、21、28天观察小鼠的体重和痛行为学表现:vonFrey细丝刺激足底测量机械缩足阈值和行走后足使用评分评价行走诱发患肢痛;分别于注射后7、14、21、28天进行X线检查评估股骨破坏程度;取股骨进行组织切片,HE染色光镜下观察肿瘤组织和骨结构破坏情况。结果:模型组小鼠体重在注射后第28天显著低于对照组和基础体重(P<0.05),注射后第7天模型组小鼠出现机械性痛觉超敏,第14天出现行走诱发患肢痛。第14天X线摄片显示有明显的骨破坏,第28天出现病理性骨折。组织学研究显示骨髓腔内肿瘤生长,向外侵蚀破坏骨皮质。结论:从疼痛行为学、放射学、组织学多方面的研究表明小鼠骨癌痛模型复制成功。To evaluate the behavior and bone destruction of the mouse model of bone cancer pain. Methods： A mouse model of bone cancer pain was developed by intrafemur inoculations of 2 × 10^5 osteolytic NCTC 2472 cells in C3hH/HeJ mice. Mechanical allodynia was evaluated by von Frey filament and ambulatory score was observed at the day of 1, 3, 5, 7, 10, 14, 21,28 after injection. The development of the bone tumor and the bone destruction were assessed by X-ray at the day of 7, 14, 21, 28 after injection. To examine the extent of bone destruction, normal and 28 d sarcoma-bearing femora were demineralized in 10% EDTA for 4 weeks and hematoxylin-eosin staining of the section to visualize the tumor infiltration. Results ： The gradual development of mechanical allodynia commenced from 7 days and low ambulatory score commenced from 14 days after inoculation of the tumor cells. The radiography of the femur showed significant bone destruction at 14 days and pathological fracture at 28 days. The bone marrow cavity was full of tumor ceils, the cortical bone was destroyed. Conclusion： Combined analysis of behavioral tests, radiology and histology results suggested that in the animal model, bone cancer pain had been copied successfully.

关 键 词：动物模型 骨癌 癌痛 触诱发痛 

分 类 号：R-332[医药卫生] R73-36