血管免疫母细胞性T细胞淋巴瘤患者血管内皮生长因子C表达的分析  

Analysis of overexpression of vascular endothelial growth factor-C in patients with angioimmunoblas- tic T-cell lymphoma

作　　者：赵维莅[1] 刘艳艳[1] Francois PLASSA 金晓龙[3] 王黎[1] Anne JANIN 沈志祥[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院血液内科,200025 [2]法国巴黎第七大学血液学研究所,法国巴黎圣路易医院 [3]上海交通大学医学院附属瑞金医院病理科,200025

出　　处：《中华血液学杂志》2007年第10期664-666,共3页Chinese Journal of Hematology

基　　金：国家高技术研究发展计划(863;2006AA02A301);教 育部留学回国人员启动基金;上海市青年科技启明星计划(05QMX1429);上海市科学技术委员会国际合作交流项目(044107025);中法先进研究计划资助项目(PRA 06-01)

摘　　要：目的探讨血管内皮生长因子 C(VEGF-C)在恶性淋巴瘤患者淋巴瘤组织中的表达及其与疾病进展的关系。方法运用实时定量 PCR 方法检测了81例恶性淋巴瘤患者淋巴瘤组织中VEGF-C 的表达。联合激光微切割技术和定量 PCR 法从淋巴瘤组织中特异性分离淋巴瘤细胞,检测淋巴瘤细胞中 VEGF-C 的表达。同时通过电镜对淋巴瘤组织切片中血管结构进行观察。结果与淋巴结反应性增生患者(8例)淋巴结的 VEGF-C 表达量(1.55±0.19)相比,血管免疫母细胞性 T 细胞淋巴瘤患者(18例)组织(15.35±9.07)和微切割的患者(10例)淋巴瘤细胞(15.19±4.28)均高表达VEGF-C(P 值分别为0.0020 和<0.01)。VEGF-C 高表达的患者常伴骨髓浸润(P=0.0039)和皮肤累及(P=0.0046),国际预后指数分组多为高危组(P=0.0302)。VEGF-C 高表达者存在血管结构异常,表现为血管内皮细胞肿胀,或缺乏周围细胞。结论 VEGF-C 表达与血管免疫母细胞性 T 细胞淋巴瘤的疾病进展密切相关。Objective To explore the vascular endothelial growth factor-C （VEGF-C） expression and its clinical significance in malignant lymphoma. Methods Lymphoma cells were isolated by laser microdissection. VEGF-C expression in lymphoma tissue and microdissected lymphoma cells was measured by realtime quantitative PCR. Meanwhile, vessel ultrastructure was identified by transmission electron microscopy. Results Comparing with that in 8 patients with reactive lymphocyte hyperplasia, VEGF-C was overexpressed in angioimmunoblastic T-cell lymphoma, both in lymphoma tissue （n = 18, P =0.0020） and in microdissected lymphoma cells （n = 10, P 〈0.0001 ）. Increased VEGF-C level was associated with bone marrow infiltration （ P = 0. 0039 ）, skin involvement （ P = 0. 0046 ） and high-risk international prognostic index （ P = 0.0302）. In VEGF-C overexpressed cases, uhrastructural study showed dystrophic vessels, with turgescent endothelial cells and absence of pericytes. Conclusion The value of VEGF-C expression might be a biomarker of disease progression in angioimmunoblastic T-cell lymphoma.

关 键 词：淋巴瘤 血管内皮生长因子 血管增生 

分 类 号：R733.1[医药卫生—肿瘤]