磷酸甘油氧化酶的菌种选育及发酵条件  

STRAIN SCREENING AND THE FERMENTATION CONDITIONS OF L-α-GLYCEROPHOSPHATE OXIDASE

作　　者：冯咏梅[1] 黎高翔[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》1997年第1期26-31,共6页Acta Microbiologica Sinica

基　　金："八五"攻关项目。

摘　　要：利用含甘油的斜面培养基连续传代、诱导筛选的方法,从大量链球菌中选育到一株产磷酸甘油氧化酶(L-α-Glycerophosphate Oxidase EC 1.1.3.21)活性较高的粪链球菌(Streptococcus faecalis) GPO 605。培养及产酶最佳条件表明,最适培养基组成(％):酪蛋白胨1,酵母提取物1.5,甘油0.2,葡萄糖0.1,K_2HPO_40.5,无机盐0.25ml,pH7.5。最适培养条件:250ml三角瓶中装100ml培养基,30℃,200r/min旋转摇床振荡培养8h。在最适培养及产酶条件下,每毫升发酵液的酶活力提高10倍以上。Streptococcus faecium GPO 605, which can produce relative high L-α-glycerophosphate oxidase, was isolated and induced from many strains of streptococcus. The culture exhibited maximum enzyme activity at 30cccccc for 8h, 200r /min, 100ml medium /250ml flask. The existence of glycerol in the medium can induce the synthesis of the enzyme efficiently and the limited glucose was indispensable to the enzyme production. Other optimum medium composition included casein, yeast extract, HPO42- and some metal ions etc.

关 键 词：磷酸甘油氧化酶 粪链球菌 菌种 选育 发酵条件 

分 类 号：Q925.9[生物学] Q920.1