油菜的遗传转化及抗溴苯腈转基因油菜的获得  

OILSEED RAPE TRANSFORMATION AND THE ESTABLISHMENT OF A BROMOXYNIL-RESISTANT TRANSGENIC OILSEED RAPE

作　　者：钟蓉[1] 朱峰[2] 刘玉乐[2] 李胜国[2] 康良仪[2] 罗鹏[1] 

机构地区：[1]四川联合大学植物研究所 [2]中国科学院微生物研究所,北京100080

出　　处：《Acta Botanica Sinica》1997年第1期22-27,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家攀登计划资助项目

摘　　要：以油菜(Brassica napus L.)的下胚轴和子叶为转化受体,建立了油菜的高效转化系统。在此基础上,将抗除草剂溴苯腈基因(bxn基因)导入油菜,获得了抗溴苯腈转基因油菜。分子检测实验证明,转基因油菜中含有bxn基因。转基因油菜可抗高达10^(-3)mol/L的溴苯腈。Using hypocotyls and cotyledons as transformed materials, an efficient transformation system of oilseed rape (Brassica napus L. ) was established. Bxn gene (bromoxynil-resistant gene) was introduced into these plants, and bromoxynil-resistant transgenic oilseed rape was obtained. The molecular monitoring experiments showed that these transgenic plants contained bxn gene. The herbicide experiments showed that these transgenic plants had resistance to 10 - mol/L bromoxynil.

关 键 词：油菜 遗传转化 抗溴苯腈 转基因油菜 基因工程 

分 类 号：S565.403.2[农业科学—作物学] S188