bcr/abl反义RNA片段对Ph+人白血病细胞系分化的影响  

THE EFFECT OF ANTISENSE TRANSCRIPTS TO BCR/ABL FUSION GENE ON DIFFERENTIATION OF PHILADELPHIA CHROMOSOME-POSITIVE LEUKEMIA CELL LINES

作　　者：郝秀娟[1] 王波[2] 李秀森[1] 奚永志[1] 江飞子[1] 毛宁[1] 杜德林[1] 唐佩弦[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]军事医学科学院附属医院

出　　处：《细胞生物学杂志》1997年第1期38-41,共4页Chinese Journal of Cell Biology

摘　　要：为探讨Ph+白血病细胞中bcr/ab1表达的降低对细胞分化的影响,本文利用脂质体介导的方法将表达bcr/ab1融合区反义RNA片段的重组质粒导入K562和BV173细胞系,以Southern和Northern杂交以及筑巢式逆转录酶-聚合酶链反应技术证实外源DNA已在靶细胞内整合与表达,且bcr/ab1反义RNA片段的表达使内源性bcr/ab1 mRNA表达水平降低。未观察到表达bcr/ab1反义RNA片段的K562细胞出现粒单系或红系分化,重组质粒转染前后BV173细胞表面的CD10和CD34抗原以及K562细胞表面的CD13和CD33抗原表达无明显变化,提示bcr/ab1反义RNA片段抑制增殖的同时未引发分化与成熟。To investigate whether Ph chromosome-positive leukemia cell lines would undergo differe-ntiation after the expression of endogenous bcr/abl fusion gene was inhibited. The recombina-nt plasmids expressing antisense transcripts to bcr/abl fusion gene were transfected into K 562 and BV 173 cell lines by lipofectin reagent. Southern and Northern blot hybridizations as well as nested RT-PCR analysis proved the integration and expression of exogenous DNA in target cells. Antisense transcripts to bcr/abl reduced the level of endogenous bcr/abl mRNA. No my-eolomonocytic and erythroid differentiations were observed in cells with antisense transcripts to bcr/abl. Also no obvious alterations occurred in the expression levels of CD 10 and CD 34 antigens in BV 173 celis and as well as CD 13 and CD 33 antigens in K 562 celis before and after transfection. It was suggested that proliferative inhibition did not always accompanying terminal differentiation.

关 键 词：白血病细胞系 BCR/ABL 反义RNA 分化 PH染色体 

分 类 号：R733.702[医药卫生—肿瘤]