福赛斯坦纳菌与牙周洁刮治疗效的关系  

Association between Tannerella forsythia and the clinical parameters following scaling and root planing assessed by TaqMan real-time PCR

作　　者：杨禾[1] 徐屹[2] 孟姝[1] 赵蕾[1] 赵寰[1] 吴亚菲[2] 

机构地区：[1]四川大学口腔生物医学工程教育部重点实验室,四川成都610041 [2]四川大学华西口腔医院牙周科

出　　处：《临床口腔医学杂志》2007年第10期605-607,共3页Journal of Clinical Stomatology

基　　金："十五"国家科技攻关计划项目(No2004BA720A26)

摘　　要：目的:分析福赛斯坦纳菌与牙周洁刮治术(SRP)疗效的关系。方法:采集20例慢性牙周炎患者(平均年龄44.33±13.86岁)SRP治疗前后口内4个区牙周袋最深位点的龈下菌斑,并记录临床指标,应用TaqMan实时荧光定量PCR法定量检测样本中的福赛斯坦纳菌。结果:SRP治疗能有效改善临床指标(P<0.001),降低福赛斯坦纳菌的检出率和数量水平(P<0.001);治疗后福赛斯坦纳菌的下降程度与PD改善呈正相关(r=0.50,P<0.001)。结论:牙周洁刮治术后福赛斯坦纳菌的下降程度可以预测牙周洁刮治术的治疗结果。TaqMan实时荧光定量PCR法能用于牙周治疗疗效的评价。Objective： The purpose of the present study was to evaluate the association between TannereUaforsythia and clinical parameters following scaling and root planing （SRP）. Method： 20 patients of periodontitis volunteered in this study （mean age 44.33±13.86）. Microbial and clinical examinations were performed at the baseline and at 3 month posttherapy. Subgingival plaque samples were taken from the deepest periodontal pockets in each segment. TaqMan real-time polymerase chain reaction was used to detect the presence and levels of Tannerella forsythia. Result：There was an improvement in the average of each clinical parameters after SRP （P〈0.001）. The prevalence and levels of Tannerella forsythia were significantly reduced after SRP （P〈0.001）. There was positive correlation between the degree of Tannerella forsythia decreased and PD improvement （r=0.50, P〈0.001）. Conclusion： The degree of TannereUaforsythia decreased might be useful for predicting the outcome of SRP. The real-time polymerase chain reaction can be used to evaluate the effect of periodontal therapy.

关 键 词：牙周炎 洁刮治术 福赛斯坦纳菌 实时荧光定量PCR 

分 类 号：R781.4[医药卫生—口腔医学] R780.2[医药卫生—临床医学]