TNF-α对体外培养人眼小梁细胞CD44s表达的影响及意义  

Effect of tumor necrosis factor-αon the expression of CD44s in cultured human trabecular meshwork cells

作　　者：陈书扬[1] 吴瑜瑜[1] 朱益华[2] 林玲[3] 

机构地区：[1]福建医科大学附属第二医院眼科 [2]福建医科大学附属第一医院 [3]福建医科大学分子医学中心

出　　处：《临床眼科杂志》2007年第5期458-461,共4页Journal of Clinical Ophthalmology

基　　金：福建省自然科学基金资助(C0310011)

摘　　要：目的探讨肿瘤坏死因子-α(TNF-α)对体外培养人眼小梁细胞CD44s表达的影响及意义。方法采用体外培养的第3代人眼小梁细胞经浓度为0.0 ng/ml(对照组)、5 ng/ml、10ng/ml、20 ng/ml的TNF-α处理24 h后,分别应用流式细胞术定量和RT-PCR半定量检测CD44s的表达量。结果浓度为5 ng/ml、10 ng/ml、20 ng/ ml的TNF-α处理组流式细胞术结果平均荧光强度分别为(7.79±9.00)、(8989±14.64)、(96.15±14.96),与对照组的(73.93±12.39)比较,有显著性差异;RT-PCR法CD44/G3PDH像素值比值分别为(0.63±0.09)、(0.79±0.12)、(0.91±0.13),与对照组比值(0.58±0.10)比较有显著性差异。结论TNF-α能够上调小梁细胞膜蛋白CD44s的表达,通过调节CD44s蛋白的表达可能会抑制原发性开角型青光眼(POAG)患者小梁细胞的丢失。Objective To investigate the effect of tumor necrosis factor-α （TNF-α） on CD44s expression in cultured human trabecular meshwork cells. Methods CD44s Expression in cultured human trabecular meshwork cells was measured by quantitative flow cytometry and semi-quantitative RT-PCR after treated with 0. 0ng/ml （control）,5ng/ml, 10ng/ml,20ng/ml TNF-α for 24h. Results The mean fluorescence intensity of CD44s expression of ceils treared with 0. 0ng/ml （control） ,5 ng/ml, 10ng/ml,20ng/ml TNF-α was 73.93 ± 12.39,76.79 ± 9.00, 89.89 ± 14.64 , 96.15 ± 14.96 ; and the value of CD44s/G3PDH of the ceils was 0.58 ±0. 100.63 ±0.09,0.79 ±0.12,0.91 ±0.13 expectivly. The difference between these treated groups and that of the control group was statistically significant. Conclusion TNF-α could increase CD44s expression in cultured human trabecular meshwork cells. Upregulation of CD44 expression through elevated levels TNF-α might inhibit loss of trabecular meshwork cells in the eyes of POAG.

关 键 词：小梁网 肿瘤坏死因子-Α 细胞培养 CD44 

分 类 号：R775[医药卫生—眼科]