皂化法提取胞内辅酶Q_(10)的研究  

Study on the Intracellular Coenzyme Q_(10) Extraction with Saponification Method

作　　者：李聚海[1] 岳田利[1] 袁亚宏[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨陵712100

出　　处：《农产品加工（下）》2007年第3期14-17,共4页Farm Products Processing

摘　　要：以根癌土壤杆菌为出发菌株,采用发酵法生产辅酶Q10,考察了破壁方法、皂化温度、皂化时间、加碱量、皂化顺序对辅酶Q10提取率的影响,试验得到皂化的最佳条件为:皂化温度60℃,皂化时间30min,皂化液与破碎液比例为5∶2。与未皂化相比,皂化后的细胞碎片经固液萃取后得到的辅酶Q10提取量提高了39.65%,并且消除了杂质对辅酶Q10测定的影响,该方法可行性强,具有广阔的发展前景。Using Agrobacterium tumefaciens as starting strain, producing Coenzyme Q10 by fermentation. The saponifcation method is applied to extracting Coenzyme Q10 in this paper, Influences of several factors such as cell-break method, saponification temperature and time, adding alkaline quantity, saponification sequence on Coenzyme Q10 extracting were studied. The best conditions of saponification were temperature 60℃, time 30min and saponification fluid ： breaking fluid=5 ： 2, After being saponification, the extracted amount of Coenzyme Q10 was about 39.65% higher than that not saponified, and eliminated the effect of imourity on determination of the content of Coenzyme Q10 in cell. The method has opened vast vistas.

关 键 词：辅酶Q10 皂化 提取 

分 类 号：Q814[生物学—生物工程]