共刺激信号B7-1表达对小鼠肿瘤细胞生长和转移的影响  

Abrogated or decreased tumorigenicity and metastasis induced by retroviral mediated B7.1 gene transfer on marine tumors with different immunogenicity

作　　者：马强中[1] 吴易 元遇珑 周颖 王洪平 张友会 

机构地区：[1]中国医学科学院肿瘤研究所肿瘤医院,中国协和医科大学肿瘤学院免疫室

出　　处：《中华医学杂志》1997年第5期374-377,共4页National Medical Journal of China

摘　　要：为Ｂ７１分子基因在肿瘤免疫基因治疗中的应用提供实验依据。方法通过逆转录病毒介导的基因转移技术，将Ｂ７１ｃＤＮＡ导入具有不同免疫原性的小鼠肿瘤细胞获得表达，并观察转基因细胞在纯系小鼠体内生长和转移的改变。结果由于Ｂ７－１基因的转导表达，使具有免疫原性的ＥＬ４Ｔ淋巴瘤细胞致瘤性丧失；使非免疫原性的Ｂ１６黑色素瘤、Ｐ８１５肥大细胞瘤和ＭＡ８９１乳腺癌细胞的致瘤性减弱；使Ｂ１６细胞的实验性转移和ＭＡ８９１细胞的自发肺转移能力降低。通过转导Ｂ７－１基因的Ｂ１６细胞的免疫接种，可使再次接种的亲本Ｂ１６细胞的致瘤性减弱：但对已生长的亲本Ｂ１６细胞影响不明显。结论Ｂ７１基因导入不同的肿瘤细胞后，可引起机体产生不同程度抗肿瘤免疫反应，使肿瘤细胞在体内的生长和转移能力丧失或减弱，这种反应的强弱与肿瘤细胞本身的特性有关。Objective To investigate the differences of tumorigenicity and metastasis of murine tumors with different immunogenicity by B7.1gene transfer.Method Using retroviral-mediated gene transfer,we transduced B7.1cDNA into a panel of murine tumor lines with different immunogenicity to study the ef fect of B7.1 costimulation on antitumor immunity.Results After transduced with B7.1 cDNA.the immunogenic T lymphoma EL4 regressed completely,and tumorgenicity of three nonimmunogenic tu mors melanoma B16,mastocytoma P815 and mammary adenocarcinoma MA891 were significantly re duced in syngeneic mice.The experimental metastasis of B16 and spontaneous pulmonary metastasis of MA891 were profoundly suppressed.Moreover,immunization with B7.1 cDNA transduced

关 键 词：B7-1 肿瘤细胞生长 肿瘤转移 基因表达 免疫疗法 

分 类 号：R730.51[医药卫生—肿瘤] R73－37[医药卫生—临床医学]