β-连环素与T细胞因子4在非小细胞肺癌中表达关系及意义  

The relationship between expressions of β-catenin and TCF-4 in non-small cell lung cancer

作　　者：王妍[1,2] 刘楠[1,2] 戴顺东[1,2] 徐洪涛[1,2] 王恩华[1,2] 

机构地区：[1]中国医科大学病理学教研室 [2]中国医科大学附属第一医院病理科,沈阳110001

出　　处：《中国肺癌杂志》2007年第5期370-375,共6页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金(No.30470764)资助~~

摘　　要：背景与目的β-连环素(β-catenin)和T细胞因子4(TCF-4)表达与非小细胞肺癌(NSCLC)临床病理因素相关,本研究的目的是分析NSCLC中β-连环素与TCF-4的关系和意义。方法应用免疫组化方法观察NSCLC中β-连环素表达情况;应用免疫荧光、免疫共沉淀、原位杂交等方法分析NSCLC中β-连环素异常定位、蓄积及与TCF-4的关系。结果NSCLC中β-catenin的蛋白和mRNA水平明显高于癌旁正常肺组织,β-连环素在NSCLC肿瘤细胞胞浆和(或)胞核异常表达率为78.74%(100/127),其中核表达率为37.01%(47/127)。β-连环素异常表达与分化程度(P=0.0008)和淋巴结转移(P=0.017)相关。TCF-4在86.67%(52/60)的肿瘤细胞核和(或)浆表达,中、低分化肺癌组织的TCF-4表达强度显著高于高分化癌组织。免疫共沉淀检测发现β-连环素和TCF-4核内共同表达的病例中有76.47%(13/17)存在β-连环素/TCF-4复合体,其中核内61.54%,浆内38.46%。结论β-连环素蛋白和 mRNA的异常表达与NSCLC的恶性表型有关。TCF-4的表达与肺癌的低分化程度相关,β-连环素/TCF-4复合体在NSCLC中较普遍存在。Background and objective It has been known that the expressions of β-catenin and T cell factor 4 （TCF-4） were associated with clinicopathological parameters in non-small cell lung cancer （NSCLC）. The objective of this study is to explore the relationship between expressions of β-catenin and TCF-4 in NSCLC. Methods The expression of β-catenin was detected with immunohistochemistry in 100 lung cancer samples; the relationship between abnormal located and preserved β-catenin with TCF-4 was investigated by immunofluorescence, co-immunoprecipitation and hybridization in situ. Results The levels of protein and mRNA were both significantly higher in NSCLC than in corresponding normal lung tissues. Aberrant cytoplasmic and/or nuclear expression of β-catenin were 78. 74% （100/127） while aberrant nuclear expression was 37.01% （47/127）. Aberrant nuclear β-catenin was significantly associated with differentiation （P=0. 0008） and lymphatic metastasis （P= 0. 017）. Positive TCF-4 expression （86.67 %, 52/60） was normally seen in nucleus and cytoplasm of cancer cells. The intensity of TCF-4 expression was significantly higher in moderately and poorly differentiated lung cancers than that in well differentiated ones. In total 17 cases of β-catenin （＋） and TCF-4 （＋） patients, 13 cases were detected with the β-catenin/TCF-4 complex, 61.54% in nucleus and 38.46% in cytoplasm. Conclusion The abnormal mRNA and protein expressions of β-catenin are associated with malignant phenotype in NSCLC. TCF-4 expression is associated with poor differentiation in lung cancers. The β-catenin/TCF-4 complex exists widely in NSCLC.

关 键 词：Β-连环素 T细胞因子4(TCF-4) β-连环素/TCF-4复合体 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]